Cas9 targeted enrichment of mobile elements using nanopore sequencing
Sign Up to like & getrecommendations! Published in 2021 at "Nature Communications"
DOI: 10.1038/s41467-021-23918-y
Abstract: Mobile element insertions (MEIs) are repetitive genomic sequences that contribute to genetic variation and can lead to genetic disorders. Targeted and whole-genome approaches using short-read sequencing have been developed to identify reference and non-reference MEIs;… read more here.
Keywords: targeted enrichment; enrichment; mobile elements; nanopore sequencing ... See more keywords
Targeted enrichment outperforms other enrichment techniques and enables more multi-species RNA-Seq analyses
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-31420-7
Abstract: Enrichment methodologies enable the analysis of minor members in multi-species transcriptomic data. We compared the standard enrichment of bacterial and eukaryotic mRNA to a targeted enrichment using an Agilent SureSelect (AgSS) capture for Brugia malayi,… read more here.
Keywords: targeted enrichment; enriched libraries; multi species; outperforms enrichment ... See more keywords
Abstract 3544: High performance multiplexed targeted enrichment sequencing from FFPE tissues
Sign Up to like & getrecommendations! Published in 2019 at "Cancer Research"
DOI: 10.1158/1538-7445.sabcs18-3544
Abstract: Library construction for Next-Generation Sequencing (NGS) using formalin-fixed paraffin-embedded (FFPE) samples offers unique challenges in acquiring high-quality sequencing data due to wide distribution of sample quality. Specifically, differences in formalin fixation methods lead to crosslinked… read more here.
Keywords: multiplexed targeted; performance; nucleic acid; performance multiplexed ... See more keywords
Phylogenomics of the Major Tropical Plant Family Annonaceae Using Targeted Enrichment of Nuclear Genes
Sign Up to like & getrecommendations! Published in 2019 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2018.01941
Abstract: Targeted enrichment and sequencing of hundreds of nuclear loci for phylogenetic reconstruction is becoming an important tool for plant systematics and evolution. Annonaceae is a major pantropical plant family with 110 genera and ca. 2,450… read more here.
Keywords: targeted enrichment; plant; family; phylogenomics major ... See more keywords
A preliminary molecular phylogeny of the family Hydroptilidae (Trichoptera): an exploration of combined targeted enrichment data and legacy sequence data
Sign Up to like & getrecommendations! Published in 2022 at "ZooKeys"
DOI: 10.3897/zookeys.1111.85361
Abstract: Abstract Hydroptilidae is an extremely diverse family within Trichoptera, containing over 2,600 known species, that displays a wide array of ecological, morphological, and habitat diversity. However, exploration into the evolutionary history of microcaddisflies based on… read more here.
Keywords: family; targeted enrichment; enrichment data; exploration ... See more keywords