Novel Nanoencapsulation Technology and its Potential Role in Bile Acid-Based Targeted Gene Delivery to the Inner Ear.
Sign Up to like & getrecommendations! Published in 2022 at "Small"
DOI: 10.1002/smll.202204986
Abstract: Hearing loss impacts a large proportion of the global population. Damage to the inner ear, in particular the sensitive hair cells, can impact individuals for the rest of their lives. There are very limited options… read more here.
Keywords: inner ear; nanoencapsulation; targeted gene; gene delivery ... See more keywords
In Situ Sequencing: A High-Throughput, Multi-Targeted Gene Expression Profiling Technique for Cell Typing in Tissue Sections.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0623-0_20
Abstract: Recent advances of image-based in situ mRNA quantification methods allow to visualize where in a tissue section a set of genes is expressed. It enables to map large numbers of genes in parallel and by… read more here.
Keywords: gene expression; throughput multi; situ sequencing; multi targeted ... See more keywords
Targeted Gene Silencing in Malignant Hematolymphoid Cells Using GapmeR.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0771-8_15
Abstract: Delivery of conventional antisense oligonucleotides or small interfering RNA (siRNA) molecules into hematolymphoid cells for targeted gene silencing has been proven to be difficult. Here, we describe a simple protocol to knockdown specific gene(s) in… read more here.
Keywords: hematolymphoid cells; cells using; gene silencing; gene ... See more keywords
Use of Auxotrophic Markers for Targeted Gene Insertions in Trichoderma reesei.
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DOI: 10.1007/978-1-0716-1048-0_5
Abstract: In this chapter, we describe a routinely used strategy for targeted gene insertions in Trichoderma reesei using auxotrophic markers. Generally, targeted gene integrations are advantageous over random, ectopic integration, because the copy number and locus… read more here.
Keywords: trichoderma reesei; auxotrophic markers; gene insertions; gene ... See more keywords
MitoTALENs: A Method for Targeted Gene Disruption in Plant Mitochondrial Genomes.
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DOI: 10.1007/978-1-0716-1653-6_22
Abstract: Modification of plant mitochondrial genomes is still a difficult task, especially in multicellular plants. Transcription activator-like effector nucleases with a mitochondrial localization signal (mitoTALENs) can cut out a desired sequence from the mitochondrial genome in… read more here.
Keywords: plant mitochondrial; mitochondrial genomes; method targeted; mitotalens method ... See more keywords
Targeted Gene Knockdown in Early Embryos Using siRNA.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6988-3_14
Abstract: RNA interference is a convenient and highly effective technique to investigate the biological function of genes. Adequately designed RNA molecules introduced into an oocyte are able to bind specific endogenous mRNAs and trigger their degradation.… read more here.
Keywords: embryos using; using sirna; early embryos; gene ... See more keywords
Creating Targeted Gene Knockouts in Brassica oleracea Using CRISPR/Cas9.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8991-1_12
Abstract: While public and political views on genetic modification (inserting "foreign" genes to elicit new traits) have resulted in limited exploitation of the technology in some parts of the world, the new era of genome editing… read more here.
Keywords: gene knockouts; creating targeted; brassica oleracea; gene ... See more keywords
Electrostatically assembled dendrimer complex with a high‐affinity protein binder for targeted gene delivery
Sign Up to like & getrecommendations! Published in 2018 at "International Journal of Pharmaceutics"
DOI: 10.1016/j.ijpharm.2018.04.015
Abstract: Graphical abstract Figure. No Caption available. Abstract Although a variety of non‐viral gene delivery systems have been developed, they still suffer from low efficiency and specificity. Herein, we present the assembly of a dendrimer complex… read more here.
Keywords: dendrimer; gene delivery; dendrimer complex; targeted gene ... See more keywords
Targeted gene capture sequencing in diagnosis of dystonia patients
Sign Up to like & getrecommendations! Published in 2018 at "Journal of the Neurological Sciences"
DOI: 10.1016/j.jns.2018.04.005
Abstract: BACKGROUND Dystonia is a movement disorder with high clinical and genetic heterogeneity. Molecular diagnosis is important for an accurate diagnosis of dystonia. Targeted gene capture sequencing has been an effective method for screening multiple candidate… read more here.
Keywords: capture sequencing; dystonia; gene; gene capture ... See more keywords
scPNMF: sparse gene encoding of single cells to facilitate gene selection for targeted gene profiling
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DOI: 10.1093/bioinformatics/btab273
Abstract: ABSTRACT: Motivation Single-cell RNA sequencing (scRNA-seq) captures whole transcriptome information of individual cells. While scRNA-seq measures thousands of genes, researchers are often interested in only dozens to hundreds of genes for a closer study. Then,… read more here.
Keywords: scrna seq; gene selection; gene profiling; gene ... See more keywords
Targeted gene panel analysis of Japanese patients with maturity‐onset diabetes of the young‐like diabetes mellitus: Roles of inactivating variants in the ABCC8 and insulin resistance genes
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Diabetes Investigation"
DOI: 10.1111/jdi.13957
Abstract: To investigate the genetic background of Japanese patients with suspected maturity‐onset diabetes of the young (MODY). read more here.
Keywords: diabetes young; maturity onset; onset diabetes; japanese patients ... See more keywords