Targeted Mutagenesis in Hexaploid Bread Wheat Using the TALEN and CRISPR/Cas Systems.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7337-8_11
Abstract: The use of sequence-specific transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeats-associated system (CRISPR/Cas9) have provided powerful reverse genetic approaches to the targeted modification of genomes in numerous organisms. Both… read more here.
Keywords: crispr; wheat; targeted mutagenesis; talen crispr ... See more keywords
CRISPR/Cas9-Mediated Targeted Mutagenesis in Wheat Doubled Haploids.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9865-4_15
Abstract: CRISPR/Cas9-based genome editing technology has the potential to revolutionize agriculture, but many plant species and/or genotypes are recalcitrant to conventional transformation methods. Additionally, the long generation time of crop plants poses a significant obstacle to… read more here.
Keywords: crispr; wheat; crispr cas9; mediated targeted ... See more keywords
An integrated protocol for targeted mutagenesis with CRISPR-Cas9 system in the pea aphid.
Sign Up to like & getrecommendations! Published in 2019 at "Insect biochemistry and molecular biology"
DOI: 10.1016/j.ibmb.2019.04.016
Abstract: CRISPR-Cas9 technology is a very efficient functional analysis tool and has been developed in several insects to edit their genome through injection of eggs with guide RNAs targeting coding sequences of genes of interest. However,… read more here.
Keywords: crispr cas9; protocol targeted; pea aphid; targeted mutagenesis ... See more keywords
Targeted mutagenesis in human iPSCs using CRISPR genome-editing tools.
Sign Up to like & getrecommendations! Published in 2021 at "Methods"
DOI: 10.1016/j.ymeth.2021.01.002
Abstract: Mutagenesis studies have rapidly evolved in the era of CRISPR genome editing. Precise manipulation of genes in human induced pluripotent stem cells (iPSCs) allows biomedical researchers to study the physiological functions of individual genes during… read more here.
Keywords: genome editing; human ipscs; targeted mutagenesis; crispr genome ... See more keywords
Computer-Aided Targeted Mutagenesis of Thermoclostridium caenicola d-Allulose 3-Epimerase for Improved Thermostability.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.1c07256
Abstract: d-Allulose 3-epimerase (DAEase) is a key enzyme in d-allulose bioproduction. DAEase from Thermoclostridium caenicola suffers from poor thermostability, hampering its large-scale applications in industry. In this study, mutants A70P, G107P, F155Y, and D162T with increased… read more here.
Keywords: improved thermostability; thermoclostridium caenicola; allulose epimerase; targeted mutagenesis ... See more keywords
EvolvR-ing to targeted mutagenesis
Sign Up to like & getrecommendations! Published in 2018 at "Nature Biotechnology"
DOI: 10.1038/nbt.4247
Abstract: 819 Directed evolution of novel molecular and cellular functions often makes use of tools for genetic diversification, such as error-prone polymerases and saturation mutagenesis. Writing in Nature, Halperin et al.1 describe a new mutagenesis tool,… read more here.
Keywords: evolvr ing; mutagenesis; targeted mutagenesis; ing targeted ... See more keywords
Targeted mutagenesis in wheat microspores using CRISPR/Cas9
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-24690-8
Abstract: CRISPR/Cas9 genome editing is a transformative technology that will facilitate the development of crops to meet future demands. However, application of gene editing is hindered by the long life cycle of many crop species and… read more here.
Keywords: wheat; crispr cas9; cas9; targeted mutagenesis ... See more keywords
Targeted mutagenesis in rabbit using an engineered BhCas12b variant
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Molecular Cell Biology"
DOI: 10.1093/jmcb/mjac076
Abstract: Dear Editor, The clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated protein (CRISPR–Cas) system has exhibited powerful abilities to manipulate genomes of animals and plants (Knott and Doudna, 2018). Up to now, numerous Cas… read more here.
Keywords: genome editing; crispr; rabbit using; mutagenesis rabbit ... See more keywords
Targeted mutagenesis of a conserved anther‐expressed P450 gene confers male sterility in monocots
Sign Up to like & getrecommendations! Published in 2017 at "Plant Biotechnology Journal"
DOI: 10.1111/pbi.12633
Abstract: Summary Targeted mutagenesis using programmable DNA endonucleases has broad applications for studying gene function in planta and developing approaches to improve crop yields. Recently, a genetic method that eliminates the need to emasculate the female… read more here.
Keywords: seed; p450 gene; targeted mutagenesis; gene ... See more keywords
Targeted mutagenesis in tetraploid switchgrass (Panicum virgatum L.) using CRISPR/Cas9
Sign Up to like & getrecommendations! Published in 2018 at "Plant Biotechnology Journal"
DOI: 10.1111/pbi.12778
Abstract: Summary The CRISPR/Cas9 system has become a powerful tool for targeted mutagenesis. Switchgrass (Panicum virgatum L.) is a high yielding perennial grass species that has been designated as a model biomass crop by the U.S.… read more here.
Keywords: crispr cas9; cas9; targeted mutagenesis; switchgrass panicum ... See more keywords
CRISPR/Cas9‐targeted mutagenesis of the OsROS1 gene induces pollen and embryo sac defects in rice
Sign Up to like & getrecommendations! Published in 2020 at "Plant Biotechnology Journal"
DOI: 10.1111/pbi.13388
Abstract: Rice yield and sustainable production are important issues for global food safety (Itoh et al., 2005). Sterility mutants are appropriate materials for understanding the molecular mechanisms underlying fertility regulation in rice, and are potential germplasm… read more here.
Keywords: rice; crispr cas9; targeted mutagenesis; osros1 gene ... See more keywords