A large‐scale targeted proteomics of plasma extracellular vesicles shows utility for prognosis prediction subtyping in colorectal cancer
Sign Up to like & getrecommendations! Published in 2022 at "Cancer Medicine"
DOI: 10.1002/cam4.5442
Abstract: The pathogenesis of cancers depends on the molecular background of each individual patient. Therefore, verifying as many biomarkers as possible and clarifying their relationships with each disease status would be very valuable. We performed a… read more here.
Keywords: plasma extracellular; extracellular vesicles; large scale; scale targeted ... See more keywords
New Approaches for Absolute Quantification of Stable‐Isotope‐Labeled Peptide Standards for Targeted Proteomics Based on a UV Active Tag
Sign Up to like & getrecommendations! Published in 2020 at "PROTEOMICS"
DOI: 10.1002/pmic.202000007
Abstract: Targeted proteomics depends on the availability of stable isotope labeled (SIL) peptide standards, which for absolute protein quantification need to be absolutely quantified. In the present study, three new approaches for absolute quantification of SIL… read more here.
Keywords: peptide standards; new approaches; targeted proteomics; stable isotope ... See more keywords
Absolute Quantitation of In Vitro Expressed Plant Membrane Proteins by Targeted Proteomics (MRM) for the Determination of Kinetic Parameters.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7411-5_15
Abstract: The purification of a functional soluble protein from biological or in vitro expression systems can be problematic and the enrichment of a functional membrane protein for biochemical analyses can be a serious technical challenge. Recently… read more here.
Keywords: absolute quantitation; quantitation vitro; vitro expressed; kinetic parameters ... See more keywords
Probing functional interactions between cytochromes P450 with principal component analysis of substrate saturation profiles and targeted proteomics.
Sign Up to like & getrecommendations! Published in 2021 at "Archives of biochemistry and biophysics"
DOI: 10.1016/j.abb.2021.108937
Abstract: We investigated the correspondence between drug metabolism routes and the composition of the P450 ensemble in human liver microsomes (HLM). As a probe, we used Coumarin 152 (C152), a fluorogenic substrate metabolized by multiple P450… read more here.
Keywords: principal component; analysis; targeted proteomics; substrate saturation ... See more keywords
A targeted proteomics approach to amyloidosis typing
Sign Up to like & getrecommendations! Published in 2018 at "Clinical Mass Spectrometry"
DOI: 10.1016/j.clinms.2018.02.001
Abstract: Abstract Background Amyloidosis is a life threatening disease caused by deposition of various types of blood serum proteins in organs and tissues. Knowing the type of protein involved is the basis of a correct diagnosis… read more here.
Keywords: proteomics approach; protein; tissue; targeted proteomics ... See more keywords
Advancing translational research and precision medicine with targeted proteomics.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of proteomics"
DOI: 10.1016/j.jprot.2018.02.021
Abstract: Remarkable advances in quantitative mass spectrometry have shifted the focus of proteomics from the characterization of protein expression profiles to detailed investigations on the spatial and temporal organization of the proteome. Demands for precision therapy… read more here.
Keywords: medicine; precision medicine; research precision; targeted proteomics ... See more keywords
Global Extraction from Parallel Reaction Monitoring to Quantify Background Peptides for Improved Normalization and Quality Control in Targeted Proteomics.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c01375
Abstract: Mass spectrometry-based targeted proteomics employs heavy isotope-labeled proteins or peptides as standards to improve accuracy and precision. The input sample amount is often determined by the total quantity of endogenous proteins or peptides, as defined… read more here.
Keywords: parallel reaction; reaction monitoring; targeted proteomics; global extraction ... See more keywords
Rational Design and Development of SARS-CoV-2 Serological Diagnostics by Immunoprecipitation-Targeted Proteomics
Sign Up to like & getrecommendations! Published in 2022 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.2c01325
Abstract: Current design of serological tests utilizes conservative immunoassay approaches and is focused on fast and convenient assay development, throughput, straightforward measurements, and affordability. Limitations of common serological assays include semiquantitative measurements, cross-reactivity, lack of reference… read more here.
Keywords: development; immunoprecipitation targeted; design; targeted proteomics ... See more keywords
Simultaneous Detection of Six Isoforms of Tau Protein in Human Cerebrospinal Fluid by Multidimensional Mass Spectrometry-Based Targeted Proteomics.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.0c00826
Abstract: Abnormal expression of Tau protein can cause the development of Alzheimer's disease (AD). So far, much evidence has demonstrated that Tau has multiple isoforms. These isoforms are suggested to have distinct physiological roles and contribute… read more here.
Keywords: targeted proteomics; spectrometry based; tau protein; mass spectrometry ... See more keywords
Systematic Protein Prioritization for Targeted Proteomics Studies through Literature Mining.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.7b00772
Abstract: There are more than 3.7 million published articles on the biological functions or disease implications of proteins, constituting an important resource of proteomics knowledge. However, it is difficult to summarize the millions of proteomics findings… read more here.
Keywords: prioritization targeted; prioritization; protein prioritization; systematic protein ... See more keywords
Combining discovery and targeted proteomics reveals a prognostic signature in oral cancer
Sign Up to like & getrecommendations! Published in 2018 at "Nature Communications"
DOI: 10.1038/s41467-018-05696-2
Abstract: Different regions of oral squamous cell carcinoma (OSCC) have particular histopathological and molecular characteristics limiting the standard tumor−node−metastasis prognosis classification. Therefore, defining biological signatures that allow assessing the prognostic outcomes for OSCC patients would be… read more here.
Keywords: oral cancer; targeted proteomics; discovery; signature ... See more keywords