Targeted RNA sequencing: A routine ancillary technique in the diagnosis of bone and soft tissue neoplasms
Sign Up to like & getrecommendations! Published in 2019 at "Genes"
DOI: 10.1002/gcc.22690
Abstract: The past decade has witnessed remarkable progress in delineating the molecular pathogenesis of many mesenchymal neoplasms. This, in large part, is attributable to the application of next‐generation sequencing. As these techniques decrease in cost, and… read more here.
Keywords: soft tissue; bone soft; tissue; rna sequencing ... See more keywords
High-content, targeted RNA-seq screening in organoids for drug discovery in colorectal cancer.
Sign Up to like & getrecommendations! Published in 2021 at "Cell reports"
DOI: 10.1016/j.celrep.2021.109026
Abstract: Organoids allow the recapitulation of intestinal homeostasis and cancerogenesis in vitro; however, RNA sequencing (RNA-seq)-based methods for drug screens are missing. We develop targeted organoid sequencing (TORNADO-seq), a high-throughput, high-content drug discovery platform that uses targeted… read more here.
Keywords: seq; high content; targeted rna; drug discovery ... See more keywords
Targeted si-RNA with liposomes and exosomes (extracellular vesicles): How to unlock the potential.
Sign Up to like & getrecommendations! Published in 2017 at "International journal of pharmaceutics"
DOI: 10.1016/j.ijpharm.2017.01.056
Abstract: The concept of RNA interference therapeutics has been initiated 18 years ago, and the main bottleneck for translation of the technology into therapeutic products remains the delivery of functional RNA molecules into the cell cytoplasm.… read more here.
Keywords: rna liposomes; exosomes extracellular; delivery; rna ... See more keywords
RNA-based high-risk HPV genotyping and identification of high-risk HPV transcriptional activity in cervical tissues
Sign Up to like & getrecommendations! Published in 2019 at "Modern Pathology"
DOI: 10.1038/s41379-019-0369-7
Abstract: Nearly all cervical cancers are initiated by a persistent infection with one of the high-risk human papillomaviruses (high-risk HPV). High-risk HPV DNA testing is highly sensitive but cannot distinguish between active, productive infections and dormant… read more here.
Keywords: risk; high risk; risk hpv; rna sequencing ... See more keywords
Targeted RNA editing in brainstem alleviates respiratory dysfunction in a mouse model of Rett syndrome
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2206053119
Abstract: Significance The ability to repair mutations in situ is a large unmet need for neurological disorders, such as Rett syndrome, a disorder due to sporadic mutations in the gene encoding Methyl CpG binding protein 2… read more here.
Keywords: rna editing; respiratory dysfunction; rett syndrome; targeted rna ... See more keywords
How successful has targeted RNA interference for hepatic fibrosis been?
Sign Up to like & getrecommendations! Published in 2018 at "Expert Opinion on Biological Therapy"
DOI: 10.1080/14712598.2018.1420775
Abstract: ABSTRACT Introduction: Exposure to toxins from the portal circulation, viral infection and by-products of metabolic activity make liver tissue prone to injury. When sustained, associated inflammation leads to activation of hepatic stellate cells (HSCs), deposition… read more here.
Keywords: fibrosis; rna interference; targeted rna; hepatic fibrosis ... See more keywords
Abstract 1764: Accurate and reproducible detection of fusions and exon skipping events in NSCLC-derived samples using a comprehensive, targeted RNA-Seq system across multiple laboratories
Sign Up to like & getrecommendations! Published in 2017 at "Cancer Research"
DOI: 10.1158/1538-7445.am2017-1764
Abstract: • Targeted RNA-Seq of expression markers in NSCLC FFPE tumors requires integrated reagents, protocols, and interpretive software that can ensure consistent results across laboratories. • In this study, a comprehensive targeted NGS system, the QuantideX®… read more here.
Keywords: skipping events; system; targeted rna; comprehensive targeted ... See more keywords
Abstract 3307: A novel targeted RNA sequencing approach for identification of aggressive prostate cancer
Sign Up to like & getrecommendations! Published in 2023 at "Cancer Research"
DOI: 10.1158/1538-7445.am2023-3307
Abstract: Introduction: Prostate cancer (PC) is a highly heterogeneous disease and can be either aggressive, requiring immediate treatment, or remain slow growing and even without treatment present no significant symptoms throughout the man’s life. Currently, there… read more here.
Keywords: prostate cancer; targeted rna; risk; novel targeted ... See more keywords
Classification and genetics of pediatric B-other Acute Lymphoblastic Leukemia by targeted RNA-sequencing.
Sign Up to like & getrecommendations! Published in 2023 at "Blood advances"
DOI: 10.1182/bloodadvances.2022009179
Abstract: Acute lymphoblastic leukemia (ALL) can be classified into different subgroups based on recurrent genetic alterations. Here, targeted RNA-sequencing was used to identify the novel subgroups of ALL in 144 B-other and 40 'classical' ALL samples.… read more here.
Keywords: rna sequencing; targeted rna; lymphoblastic leukemia; acute lymphoblastic ... See more keywords
Detection of leukemia gene fusions by targeted RNA-sequencing in routine diagnostics
Sign Up to like & getrecommendations! Published in 2020 at "BMC Medical Genomics"
DOI: 10.1186/s12920-020-00739-4
Abstract: Background We have evaluated an NGS-based method to detect recurrent gene fusions of diagnostic and prognostic importance in hematological malignancies. Our goal was to achieve a highly specific assay with a simple workflow, short turnaround… read more here.
Keywords: gene fusions; rna sequencing; targeted rna; gene ... See more keywords
Mapping actionable pathways and mutations in brain tumours using targeted RNA next generation sequencing
Sign Up to like & getrecommendations! Published in 2019 at "Acta Neuropathologica Communications"
DOI: 10.1186/s40478-019-0826-z
Abstract: Many biology-based precision drugs are available that neutralize aberrant molecular pathways in cancer. Molecular heterogeneity and the lack of reliable companion diagnostic biomarkers for many drugs makes targeted treatment of cancer inaccurate for many individuals.… read more here.
Keywords: targeted rna; next generation; mapping actionable; rna next ... See more keywords