Genome editing in Streptococcus mutans through self‐targeting CRISPR arrays
Sign Up to like & getrecommendations! Published in 2018 at "Molecular Oral Microbiology"
DOI: 10.1111/omi.12247
Abstract: Streptococcus mutans is the primary etiological agent of human dental caries. Its major virulence factors, glucosyltransferases (Gtfs), utilize sucrose to synthesize extracellular polysaccharides (EPS), leading to the formation of dental plaque biofilm. The current study… read more here.
Keywords: crispr; targeting crispr; self targeting; crispr arrays ... See more keywords
Shutting down RNA-targeting CRISPR
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DOI: 10.1126/science.abc8243
Abstract: The discovery of an anti-CRISPR reveals viral escape from CRISPR immunity Explorations of the evolutionary arms race between bacteria and bacteriophages (viruses that infect bacteria) have unearthed a variety of defense mechanisms that include CRISPR-Cas… read more here.
Keywords: crispr; shutting rna; targeting crispr; rna targeting ... See more keywords
Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying functional circRNAs
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DOI: 10.1186/s13059-021-02263-9
Abstract: Short hairpin RNAs (shRNAs) are used to deplete circRNAs by targeting back-splicing junction (BSJ) sites. However, frequent discrepancies exist between shRNA-mediated circRNA knockdown and the corresponding biological effect, querying their robustness. By leveraging CRISPR/Cas13d tool… read more here.
Keywords: functional circrnas; optimized rna; rna targeting; crispr cas13d ... See more keywords
RNA-Targeting CRISPR–Cas Systems and Their Applications
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DOI: 10.3390/ijms21031122
Abstract: Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)–CRISPR-associated (Cas) systems have revolutionized modern molecular biology. Numerous types of these systems have been discovered to date. Many CRISPR–Cas systems have been used as a backbone for the… read more here.
Keywords: crispr cas; cas; cas systems; targeting crispr ... See more keywords