Generation of OCT4-EGFP, NANOG-tdTomato dual reporter human induced pluripotent stem cell line, KKUi001-A, using the CRISPR/Cas9 system.
Sign Up to like & getrecommendations! Published in 2020 at "Stem cell research"
DOI: 10.1016/j.scr.2020.101943
Abstract: OCT4 and NANOG are core transcription factor genes in self-renewal, differentiation, and reprogramming. Here, we generated an OCT4-EGFP, NANOG-tdTomato dual reporter hiPSC line, KKUi001-A, on the basis of human induced pluripotent stem cells using CRISPR/Cas9… read more here.
Keywords: reporter; oct4 egfp; line; tdtomato ... See more keywords
Evidence for Minimal Cardiogenic Potential of Stem Cell Antigen 1–Positive Cells in the Adult Mouse Heart
Sign Up to like & getrecommendations! Published in 2018 at "Circulation"
DOI: 10.1161/circulationaha.118.035273
Abstract: De novo cardiomyogenesis is limited to ≈1% per year in the adult mammalian heart.1 Whether newly formed cardiomyocytes are derived from division of pre-existing myocytes or from differentiation of resident cardiac progenitor cells is a… read more here.
Keywords: adult; heart; phd; expressing cells ... See more keywords
Expression of tdTomato and luciferase in a murine lung cancer alters the growth and immune microenvironment of the tumor
Sign Up to like & getrecommendations! Published in 2021 at "PLoS ONE"
DOI: 10.1371/journal.pone.0254125
Abstract: Imaging techniques based on fluorescence and bioluminescence have been important tools in visualizing tumor progression and studying the effect of drugs and immunotherapies on tumor immune microenvironment in animal models of cancer. However, transgenic expression… read more here.
Keywords: tdtomato; tdtomato luc; expression; immune microenvironment ... See more keywords
Molecular and Electrophysiological Characterization of Dorsal Horn Neurons in a GlyT2-iCre-tdTomato Mouse Line
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Pain Research"
DOI: 10.2147/jpr.s296940
Abstract: Purpose Spinal glycinergic neurons function as critical elements of a spinal gate for pain and itch. We have recently documented that spinal PKCγ+ neurons receive the feedforward inhibitory input driven by Aβ primary afferent. The… read more here.
Keywords: glyt2 tdtomato; glyt2; mice; glycinergic neurons ... See more keywords
T-cell calcium dynamics visualized in a ratiometric tdTomato-GCaMP6f transgenic reporter mouse
Sign Up to like & getrecommendations! Published in 2017 at "eLife"
DOI: 10.7554/elife.32417
Abstract: Calcium is an essential cellular messenger that regulates numerous functions in living organisms. Here, we describe development and characterization of ‘Salsa6f’, a fusion of GCaMP6f and tdTomato optimized for cell tracking while monitoring cytosolic Ca2+,… read more here.
Keywords: reporter mouse; gcamp6f; tdtomato; salsa6f ... See more keywords