Development of a massively parallel, genotyping-by-sequencing assay in American badger (Taxidea taxus) highlights the need for careful validation when working with low template DNA
Sign Up to like & getrecommendations! Published in 2020 at "Conservation Genetics Resources"
DOI: 10.1007/s12686-020-01146-8
Abstract: Non-invasive DNA sampling to identify and enumerate species is critical to population monitoring and for developing effective management strategies. However, individual DNA identification is often limited by degraded and low template DNA (LT-DNA) that routinely… read more here.
Keywords: parallel genotyping; dna; massively parallel; low template ... See more keywords
Preparation of Polymerase Chain Reaction Template DNA from Mouse Tail Tissue.
Sign Up to like & getrecommendations! Published in 2019 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot092700
Abstract: A simple cell or tissue lysate can provide a sufficient quality and amount of template DNA for polymerase chain reaction (PCR). In this protocol, a small piece from the tip of the tail is removed… read more here.
Keywords: template dna; polymerase chain; chain reaction; tissue ... See more keywords
Inferring the Number of Contributors to Complex DNA Mixtures Using Three Methods: Exploring the Limits of Low‐Template DNA Interpretation
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DOI: 10.1111/1556-4029.13284
Abstract: In forensic DNA casework, the interpretation of an evidentiary profile may be dependent upon the assumption on the number of individuals from whom the evidence arose. Three methods of inferring the number of contributors—NOCIt, maximum… read more here.
Keywords: three methods; dna; number; template dna ... See more keywords