Induced intra- and intermolecular template switching as a therapeutic mechanism against RNA viruses.
Sign Up to like & getrecommendations! Published in 2021 at "Molecular cell"
DOI: 10.1016/j.molcel.2021.10.003
Abstract: Viral RNA-dependent RNA polymerases (RdRps) are a target for broad-spectrum antiviral therapeutic agents. Recently, we demonstrated that incorporation of the T-1106 triphosphate, a pyrazine-carboxamide ribonucleotide, into nascent RNA increases pausing and backtracking by the poliovirus… read more here.
Keywords: intra intermolecular; template switching; pyrazine carboxamide; intermolecular template ... See more keywords
Protein innovation through template switching in the Saccharomyces cerevisiae lineage
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-01736-y
Abstract: DNA polymerase template switching between short, non-identical inverted repeats (IRs) is a genetic mechanism that leads to the homogenization of IR arms and to IR spacer inversion, which cause multinucleotide mutations (MNMs). It is unknown… read more here.
Keywords: saccharomyces cerevisiae; evolution; template switching; template ... See more keywords
Thousands of human mutation clusters are explained by short-range template switching
Sign Up to like & getrecommendations! Published in 2022 at "Genome Research"
DOI: 10.1101/2021.11.26.470150
Abstract: Variation within human genomes is unevenly distributed, and variants show spatial clustering. DNA- replication-related template switching is a poorly known mutational mechanism capable of causing major chromosomal rearrangements as well as creating short inverted sequence… read more here.
Keywords: short read; template; template switching; template switch ... See more keywords
Template-switching artifacts resemble alternative polyadenylation
Sign Up to like & getrecommendations! Published in 2019 at "BMC Genomics"
DOI: 10.1186/s12864-019-6199-7
Abstract: BackgroundAlternative polyadenylation is commonly examined using cDNA sequencing, which is known to be affected by template-switching artifacts. However, the effects of such template-switching artifacts on alternative polyadenylation are generally disregarded, while alternative polyadenylation artifacts are… read more here.
Keywords: template switching; switching artifacts; polyadenylation; cdna sequencing ... See more keywords
Interchromosomal template-switching as a novel molecular mechanism for imprinting perturbations associated with Temple syndrome
Sign Up to like & getrecommendations! Published in 2019 at "Genome Medicine"
DOI: 10.1186/s13073-019-0633-y
Abstract: BackgroundIntrachromosomal triplications (TRP) can contribute to disease etiology via gene dosage effects, gene disruption, position effects, or fusion gene formation. Recently, post-zygotic de novo triplications adjacent to copy-number neutral genomic intervals with runs of homozygosity… read more here.
Keywords: template switching; mechanism; temple syndrome; template ... See more keywords
Stimulation of Replication Template-Switching by DNA-Protein Crosslinks
Sign Up to like & getrecommendations! Published in 2018 at "Genes"
DOI: 10.3390/genes10010014
Abstract: Covalent DNA protein crosslinks (DPCs) are common lesions that block replication. We examine here the consequence of DPCs on mutagenesis involving replicational template-switch reactions in Escherichia coli. 5-Azacytidine (5-azaC) is a potent mutagen for template-switching.… read more here.
Keywords: protein crosslinks; template switching; dna; dna protein ... See more keywords