Enhancing the expression of terminal deoxynucleotidyl transferases by N‐terminal truncation
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DOI: 10.1002/biot.202400226
Abstract: Terminal deoxynucleotidyl transferase (TdT), a unique DNA polymerase that catalyzes the template‐free incorporation of nucleotides into single‐stranded DNA, has facilitated the development of various oligonucleotide‐based tools and methods, especially in the field of template‐free enzymatic… read more here.
Keywords: enhancing expression; terminal deoxynucleotidyl; truncation; terminal truncation ... See more keywords
Terminal deoxynucleotidyl transferase and CD84 identify human multi-potent lymphoid progenitors
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DOI: 10.1038/s41467-024-49883-w
Abstract: Lymphoid specification in human hematopoietic progenitors is not fully understood. To better associate lymphoid identity with protein-level cell features, we conduct a highly multiplexed single-cell proteomic screen on human bone marrow progenitors. This screen identifies… read more here.
Keywords: terminal deoxynucleotidyl; deoxynucleotidyl transferase; multi; cd84 ... See more keywords
Terminal deoxynucleotidyl transferase-mediated formation of protein binding polynucleotides
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DOI: 10.1093/nar/gkaa1263
Abstract: Abstract Terminal deoxynucleotidyl transferase (TdT) enzyme plays an integral part in the V(D)J recombination, allowing for the huge diversity in expression of immunoglobulins and T-cell receptors within lymphocytes, through their unique ability to incorporate single… read more here.
Keywords: protein; transferase mediated; deoxynucleotidyl transferase; mediated formation ... See more keywords
Evolving a terminal deoxynucleotidyl transferase for commercial enzymatic DNA synthesis
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DOI: 10.1093/nar/gkaf115
Abstract: Abstract Enzymatic DNA synthesis, using stepwise nucleotide addition catalyzed by template-independent polymerases, promises higher efficiency, quality, and sustainability than today’s industry-standard phosphoramidite-based processes. We report the directed evolution of a terminal deoxynucleotidyl transferase that uses… read more here.
Keywords: terminal deoxynucleotidyl; deoxynucleotidyl transferase; dna synthesis; enzymatic dna ... See more keywords