YESS 2.0, a Tunable Platform for Enzyme Evolution, Yields Highly Active TEV Protease Variants.
Sign Up to like & getrecommendations! Published in 2021 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.0c00452
Abstract: Here we describe YESS 2.0, a highly versatile version of the yeast endoplasmic sequestration screening (YESS) system suitable for engineering and characterizing protein/peptide modifying enzymes such as proteases with desired new activities. By incorporating features… read more here.
Keywords: yess tunable; tev protease; tev; etev ... See more keywords
Directed evolution improves the catalytic efficiency of TEV protease
Sign Up to like & getrecommendations! Published in 2019 at "Nature methods"
DOI: 10.1038/s41592-019-0665-7
Abstract: Tobacco etch virus protease (TEV) is one of the most widely used proteases in biotechnology because of its exquisite sequence specificity. A limitation, however, is its slow catalytic rate. We developed a generalizable yeast-based platform… read more here.
Keywords: directed evolution; tev protease; protease; evolution improves ... See more keywords