Chemoenzymatic Defucosylation of Therapeutic Antibodies for Enhanced Effector Functions Using Bacterial α-Fucosidases.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8648-4_19
Abstract: Core fucosylation plays a critical role in modulating the effector functions of therapeutic antibodies such as the antibody-dependent cellular cytotoxicity (ADCC) through adversely affecting the affinity of antibodies for Fcγ receptors. Thus, a facile method… read more here.
Keywords: therapeutic antibodies; bacterial fucosidases; herceptin; effector functions ... See more keywords
Therapeutic antibodies against cancer stem cells: a promising approach
Sign Up to like & getrecommendations! Published in 2017 at "Cancer Immunology, Immunotherapy"
DOI: 10.1007/s00262-017-2049-0
Abstract: Monoclonal antibodies have been extensively used to treat malignancy along with routine chemotherapeutic drugs. Chemotherapy for metastatic cancer has not been successful in securing long-term remission of disease. This is in part due to the… read more here.
Keywords: therapeutic antibodies; cancer stem; approach; cancer ... See more keywords
Sedimentation velocity analytical ultracentrifugation for characterization of therapeutic antibodies
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Reviews"
DOI: 10.1007/s12551-017-0374-3
Abstract: Sedimentation velocity analytical ultracentrifugation (SV-AUC) coupled with direct computational fitting of the observed concentration profiles (sedimentating boundary) have been developed and widely used for the characterization of macromolecules and nanoparticles in solution. In particular, size… read more here.
Keywords: therapeutic antibodies; velocity analytical; sedimentation velocity; analytical ultracentrifugation ... See more keywords
“Magic Bullets” at the center stage of immune therapy: a special issue on therapeutic antibodies
Sign Up to like & getrecommendations! Published in 2017 at "Protein & Cell"
DOI: 10.1007/s13238-017-0488-1
Abstract: Therapeutic antibodies represent one of the most significant advances in the history of medicine. Progress in the antibody therapy field was initially slow and intermittent. The first therapeutic antibody, murine-derived Murononab OKT3 for acute organ… read more here.
Keywords: medicine; therapeutic antibodies; antibody; chemistry ... See more keywords
The state‐of‐play and future of antibody therapeutics☆
Sign Up to like & getrecommendations! Published in 2017 at "Advanced Drug Delivery Reviews"
DOI: 10.1016/j.addr.2016.11.004
Abstract: Abstract It has been over four decades since the development of monoclonal antibodies (mAbs) using a hybridoma cell line was first reported. Since then more than thirty therapeutic antibodies have been marketed, mostly as oncology,… read more here.
Keywords: therapeutic antibodies; antibody; play future; future antibody ... See more keywords
Sequence features of variable region determining physicochemical properties and polyreactivity of therapeutic antibodies.
Sign Up to like & getrecommendations! Published in 2019 at "Molecular immunology"
DOI: 10.1016/j.molimm.2019.06.012
Abstract: Therapeutic antibodies have transformed the clinical practice. Not surprisingly, development of antibody therapeutics is currently the main focus of the biotechnology industry. Nonetheless, the development process is complex, and many antibodies do not reach the… read more here.
Keywords: therapeutic antibodies; features variable; polyreactivity; variable region ... See more keywords
Tractable Method for Rapid Quality Assessment of Therapeutic Antibodies in Harvested Cell Culture Fluid based on FcγRIIIa-Immobilized Magnetic Microspheres.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c01350
Abstract: FcγRIIIa-binding affinity is one of the key factors to ensure the efficacy of many antitumor therapeutic antibodies, which should be monitored along with the titer, protein aggregation, and other critical quality attributes. The conventional workflow… read more here.
Keywords: therapeutic antibodies; quality; quality assessment; riiia immobilized ... See more keywords
Recent advances in nanotechnology-based COVID-19 vaccines and therapeutic antibodies.
Sign Up to like & getrecommendations! Published in 2022 at "Nanoscale"
DOI: 10.1039/d1nr03831a
Abstract: COVID-19 has caused a global pandemic and millions of deaths. It is imperative to develop effective countermeasures against the causative viral agent, SARS-CoV-2 and its many variants. Vaccines and therapeutic antibodies are the most effective… read more here.
Keywords: covid vaccines; covid; nanotechnology; therapeutic antibodies ... See more keywords
Looking for therapeutic antibodies in next-generation sequencing repositories
Sign Up to like & getrecommendations! Published in 2019 at "mAbs"
DOI: 10.1080/19420862.2019.1633884
Abstract: ABSTRACT Recently it has become possible to query the great diversity of natural antibody repertoires using next-generation sequencing (NGS). These methods are capable of producing millions of sequences in a single experiment. Here we compare… read more here.
Keywords: therapeutic antibodies; looking therapeutic; next generation; generation sequencing ... See more keywords
In silico prediction of post-translational modifications in therapeutic antibodies
Sign Up to like & getrecommendations! Published in 2022 at "mAbs"
DOI: 10.1080/19420862.2021.2023938
Abstract: ABSTRACT Monoclonal antibodies are susceptible to chemical and enzymatic modifications during manufacturing, storage, and shipping. Deamidation, isomerization, and oxidation can compromise the potency, efficacy, and safety of therapeutic antibodies. Recently, in silico tools have been… read more here.
Keywords: therapeutic antibodies; translational modifications; prediction post; post translational ... See more keywords
Binding mechanisms of therapeutic antibodies to human CD20
Sign Up to like & getrecommendations! Published in 2020 at "Science"
DOI: 10.1126/science.abb8008
Abstract: Strength in numbers Human cluster of differentiation 20 (CD20) is expressed on malignant B cells and is the target of therapeutic antibodies used in cancer immunotherapy. Kumar et al. now present structures that explain why… read more here.
Keywords: therapeutic antibodies; type antibodies; generation; type ... See more keywords