Therapeutic protein purity and fragmented species characterization by capillary electrophoresis sodium dodecyl sulfate using systematic hybrid cleavage and forced degradation
Sign Up to like & getrecommendations! Published in 2019 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-019-01942-8
Abstract: AbstractPositive identification of capillary electrophoresis-sodium dodecyl sulfate (CE-SDS) electropherogram peaks provides information to understand protein molecular characteristics at the structural level. It is critical in the design of a robust assay that can accurately resolve,… read more here.
Keywords: capillary electrophoresis; dodecyl sulfate; electrophoresis sodium; sodium dodecyl ... See more keywords
Alginate/chitosan microparticles for gastric passage and intestinal release of therapeutic protein nanoparticles
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Controlled Release"
DOI: 10.1016/j.jconrel.2018.12.017
Abstract: Abstract Enzymes with intracellular activity have significant potential to treat diseases. Protein nanoparticles (NPs) considerably enhance intracellular delivery of enzymes. We have previously shown that a Salmonella effector enzyme, AvrA, delivered by NPs is capable… read more here.
Keywords: protein; alginate chitosan; protein nps; protein nanoparticles ... See more keywords
A fast and simple approach to optimize the unit operation high pressure homogenization - a case study for a soluble therapeutic protein in E. coli
Sign Up to like & getrecommendations! Published in 2019 at "Preparative Biochemistry and Biotechnology"
DOI: 10.1080/10826068.2018.1536988
Abstract: Abstract Escherichia coli is one of the most commonly used host organisms for the production of recombinant biopharmaceuticals. E. coli is usually characterized by fast growth on cheap media and high productivity, but one drawback… read more here.
Keywords: soluble therapeutic; product; study; pressure homogenization ... See more keywords
A Cross Company Perspective on the Assessment of Therapeutic Protein Biotransformation
Sign Up to like & getrecommendations! Published in 2022 at "Drug Metabolism and Disposition"
DOI: 10.1124/dmd.121.000462
Abstract: Unlike with new chemical entities, the biotransformation of therapeutic proteins (TPs) has not been routinely investigated or included in regulatory filings. Nevertheless, there is an expanding pool of evidence suggesting that a more in-depth understanding… read more here.
Keywords: assessment; development; biotransformation; tps ... See more keywords
Recent advances in (therapeutic protein) drug development
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DOI: 10.12688/f1000research.9970.1
Abstract: Therapeutic protein drugs are an important class of medicines serving patients most in need of novel therapies. Recently approved recombinant protein therapeutics have been developed to treat a wide variety of clinical indications, including cancers,… read more here.
Keywords: drug development; drug; therapeutic protein; protein drug ... See more keywords
Macromolecular confinement of therapeutic protein in polymeric particles for controlled release: insulin as a case study
Sign Up to like & getrecommendations! Published in 2017 at "Brazilian Journal of Pharmaceutical Sciences"
DOI: 10.1590/s2175-97902017000216039
Abstract: Sustained release systems for therapeutic proteins have been widely studied targeting to improve the action of these drugs. Molecular entrapping of proteins is particularly challenging due to their conformational instability. We have developed a micro-structured… read more here.
Keywords: insulin; macromolecular confinement; study; therapeutic protein ... See more keywords
Sophisticated Cloning, Fermentation, and Purification Technologies for an Enhanced Therapeutic Protein Production: A Review
Sign Up to like & getrecommendations! Published in 2017 at "Frontiers in Pharmacology"
DOI: 10.3389/fphar.2017.00419
Abstract: The protein productions strategies are crucial towards the development of application based research and elucidating the novel purification strategies for industrial production. Currently, there are few innovative avenues are studies for cloning, upstream, and purification… read more here.
Keywords: production; purification technologies; fermentation purification; therapeutic protein ... See more keywords