PACTS-Assisted Thermal Proteome Profiling for Use in Identifying Peptide-Interacting Proteins.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c00581
Abstract: Bioactive peptides play important roles in various biological processes. However, the traditional methods for profiling the peptide-interacting proteins require modifications to the peptide molecules, often leading to false identifications. We found that the interaction between… read more here.
Keywords: thermal proteome; pacts assisted; interacting proteins; peptide interacting ... See more keywords
Multi-comparative Thermal Proteome Profiling Uncovers New O-GlcNAc Proteins in a System-wide Method.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c03371
Abstract: Among diverse protein post-translational modifications, O-GlcNAcylation, a simple but essential monosaccharide modification, plays crucial roles in cellular processes and is closely related to various diseases. Despite its ubiquity in cells, properties of low stoichiometry and… read more here.
Keywords: multi comparative; system wide; comparative thermal; thermal proteome ... See more keywords
Precipitate-Supported Thermal Proteome Profiling Coupled with Deep Learning for Comprehensive Screening of Drug Target Proteins.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.1c00936
Abstract: Although thermal proteome profiling (TPP) acts as a popular modification-free approach for drug target deconvolution, some key problems are still limiting screening sensitivity. In the prevailing TPP workflow, only the soluble fractions are analyzed after… read more here.
Keywords: drug target; thermal proteome; drug; precipitate supported ... See more keywords
Thermal Proteome Profiling Reveals the O-GlcNAc-Dependent Meltome.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.1c10621
Abstract: Posttranslational modifications alter the biophysical properties of proteins and thereby influence cellular physiology. One emerging manner by which such modifications regulate protein functions is through their ability to perturb protein stability. Despite the increasing interest… read more here.
Keywords: thermal proteome; glcnac dependent; proteome profiling; protein ... See more keywords
Cell surface thermal proteome profiling tracks perturbations and drug targets on the plasma membrane
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DOI: 10.1038/s41592-020-01022-1
Abstract: Numerous drugs and endogenous ligands bind to cell surface receptors leading to modulation of downstream signaling cascades and frequently to adaptation of the plasma membrane proteome. In-depth analysis of dynamic processes at the cell surface… read more here.
Keywords: cell surface; surface thermal; plasma membrane; thermal proteome ... See more keywords
Meltome atlas—thermal proteome stability across the tree of life
Sign Up to like & getrecommendations! Published in 2020 at "Nature Methods"
DOI: 10.1038/s41592-020-0801-4
Abstract: We have used a mass spectrometry-based proteomic approach to compile an atlas of the thermal stability of 48,000 proteins across 13 species ranging from archaea to humans and covering melting temperatures of 30–90 °C. Protein sequence,… read more here.
Keywords: meltome atlas; atlas thermal; proteome stability; stability ... See more keywords
Thermal proteome profiling reveals Haemonchus orphan protein HCO_011565 as a target of the nematocidal small molecule UMW-868
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Pharmacology"
DOI: 10.3389/fphar.2022.1014804
Abstract: Parasitic roundworms (nematodes) cause destructive diseases, and immense suffering in humans and other animals around the world. The control of these parasites relies heavily on anthelmintic therapy, but treatment failures and resistance to these drugs… read more here.
Keywords: small molecule; umw 868; hco 011565; thermal proteome ... See more keywords