Thermodynamic activity‐based intrinsic enzyme kinetic sheds light on enzyme–solvent interactions
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DOI: 10.1002/btpr.2401
Abstract: The reaction medium has major impact on biocatalytic reaction systems and on their economic significance. To allow for tailored medium engineering, thermodynamic phenomena, intrinsic enzyme kinetics, and enzyme–solvent interactions have to be discriminated. To this… read more here.
Keywords: enzyme solvent; thermodynamic activity; intrinsic enzyme; activity based ... See more keywords
Thermodynamic Activity-Based Progress Curve Analysis in Enzyme Kinetics.
Sign Up to like & getrecommendations! Published in 2018 at "Trends in biotechnology"
DOI: 10.1016/j.tibtech.2017.10.004
Abstract: Macrokinetic Michaelis-Menten models based on thermodynamic activity provide insights into enzyme kinetics because they separate substrate-enzyme from substrate-solvent interactions. Kinetic parameters are estimated from experimental progress curves of enzyme-catalyzed reactions. Three pitfalls are discussed: deviations… read more here.
Keywords: progress; thermodynamic activity; activity based; enzyme kinetics ... See more keywords