Thirty-degree shift in optimum temperature of a thermophilic lipase by a single-point mutation: effect of serine to threonine mutation on structural flexibility
Sign Up to like & getrecommendations! Published in 2017 at "Molecular and Cellular Biochemistry"
DOI: 10.1007/s11010-017-2950-z
Abstract: In order to understand the molecular basis of cold adaptation, we have used directed evolution to transform a thermophilic lipase LipR1 into its psychrophilic counterpart. A single round of random mutagenesis followed by screening for… read more here.
Keywords: point mutation; flexibility; lipase; thermophilic lipase ... See more keywords
Biocatalysis at Extreme Temperatures: Enantioselective Synthesis of both Enantiomers of Mandelic Acid by Transesterification Catalyzed by a Thermophilic Lipase in Ionic Liquids at 120 °C
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DOI: 10.3390/catal10091055
Abstract: The use of biocatalysts in organic chemistry for catalyzing chemo-, regio- and stereoselective transformations has become an usual tool in the last years, both at lab and industrial scale. This is not only because of… read more here.
Keywords: enantiomers mandelic; mandelic acid; lipase; thermophilic lipase ... See more keywords