Effects of the substituted amino acid residues on the thermal properties of monomeric isocitrate dehydrogenases from a psychrophilic bacterium, Psychromonas marina, and a mesophilic bacterium, Azotobacter vinelandii
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DOI: 10.1007/s00792-019-01137-0
Abstract: A cold-adapted monomeric isocitrate dehydrogenase from a psychrophilic bacterium, Psychromonas marina (PmIDH), showed a high degree of amino acid sequential identity (64%) to a mesophilic one from a mesophilic bacterium, Azotobacter vinelandii (AvIDH). In this… read more here.
Keywords: bacterium; amino acid; specific activity; thermostability activity ... See more keywords
Sandwich fusion of CBM9_2 to enhance xylanase thermostability and activity.
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DOI: 10.1016/j.ijbiomac.2018.05.199
Abstract: Used as model for sandwich fusion, a mesophilic Aspergillus niger GH11 xylanase (Xyn) was fused into C2-Xyn-C2 with a thermophilic Thermotaga maritima GH10 xylanase carbohydrate-binding module CBM9_2 (C2). Linearized plasmids C2-pET20b-C2-Xyn were amplified from template… read more here.
Keywords: xylanase; sandwich fusion; thermostability activity; fusion ... See more keywords