Dimerization of Proline Dehydrogenase from Thermus thermophilus Is Crucial for Its Thermostability.
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology journal"
DOI: 10.1002/biot.201800540
Abstract: Thermus thermophilus proline dehydrogenase ( TtProDH) catalyzes the first step in proline catabolism. The thermostable flavoenzyme consists of a distorted triosephosphate isomerase (TIM) barrel and three N-terminal helices: αA, αB, and αC. Using maltose-binding protein… read more here.
Keywords: proline dehydrogenase; dimerization; thermostability; thermus ... See more keywords
The Peculiar Case of the Hyper‐thermostable Pyrimidine Nucleoside Phosphorylase from Thermus thermophilus **
Sign Up to like & getrecommendations! Published in 2020 at "Chembiochem"
DOI: 10.1002/cbic.202000679
Abstract: The poor solubility of many nucleosides and nucleobases in aqueous solution demands harsh reaction conditions (base, heat, cosolvent) in nucleoside phosphorylase‐catalyzed processes to facilitate substrate loading beyond the low millimolar range. This, in turn, requires… read more here.
Keywords: pyrimidine; thermus thermophilus; pyrimidine nucleoside; phosphorylase thermus ... See more keywords
NMR resonance assignments for the GSPII-C domain of the PilF ATPase from Thermus thermophilus in complex with c-di-GMP.
Sign Up to like & getrecommendations! Published in 2019 at "Biomolecular NMR assignments"
DOI: 10.1007/s12104-019-09906-w
Abstract: The natural transformation system of the thermophilic bacterium Thermus thermophilus is one of the most efficient DNA transport systems in terms of DNA uptake rate and promiscuity. The DNA transporter of T. thermophilus plays an… read more here.
Keywords: atpase; gmp; thermus thermophilus; thermophilus ... See more keywords
Insight into the transition between the open and closed conformations of Thermus thermophilus carboxypeptidase.
Sign Up to like & getrecommendations! Published in 2017 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2017.01.167
Abstract: Carboxypeptidase cleaves the C-terminal amino acid residue from proteins and peptides. Here, we report the functional and structural characterizations of carboxypeptidase belonging to the M32 family from the thermophilic bacterium Thermus thermophilus HB8 (TthCP). TthCP… read more here.
Keywords: open closed; carboxypeptidase; thermus thermophilus; closed states ... See more keywords
Characterization of two 2-isopropylmalate synthase homologs from Thermus thermophilus HB27.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2018.05.013
Abstract: 2-Isopropylmalate synthase (IPMS) catalyzes the first step of leucine biosynthesis and is regulated via feedback inhibition by leucine. The thermophilic bacterium, Thermus thermophilus HB27, has two IPMS homologous genes: TTC0847 and TTC0849, both of which… read more here.
Keywords: synthase; thermophilus hb27; thermus thermophilus; characterization two ... See more keywords
Effect of C-terminal domain truncation of Thermus thermophilus trehalose synthase on its substrate specificity.
Sign Up to like & getrecommendations! Published in 2017 at "Enzyme and microbial technology"
DOI: 10.1016/j.enzmictec.2016.10.006
Abstract: The C-terminal domain of the three-domain-comprising trehalose synthase from Thermus thermophilus was truncated in order to study the effect on the enzyme's activity and substrate specificity. Compared with the wild-type (WT) enzyme, the two truncated… read more here.
Keywords: terminal domain; thermus thermophilus; trehalose synthase; domain ... See more keywords
Heterologous expression and characterization of a novel subtilisin-like protease from a thermophilic Thermus thermophilus HB8.
Sign Up to like & getrecommendations! Published in 2019 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2019.07.101
Abstract: Subtilisins are a family of serine proteases used widely throughout the detergent, leather and food industries, with the identification and development of new enzymes holding much potential value. Thermus thermophilus HB8 was examined for serine… read more here.
Keywords: mature subtilisin; thermus thermophilus; thermophilus hb8; subtilisin ... See more keywords
Proteins from Thermophilic Thermus thermophilus Often Do Not Fold Correctly in a Mesophilic Expression System Such as Escherichia coli
Sign Up to like & getrecommendations! Published in 2022 at "ACS Omega"
DOI: 10.1021/acsomega.2c04786
Abstract: Majority of protein structure studies use Escherichia coli (E. coli) and other model organisms as expression systems for other species’ genes. However, protein folding depends on cellular environment factors, such as chaperone proteins, cytoplasmic pH,… read more here.
Keywords: escherichia coli; thermus thermophilus; expression system; expression ... See more keywords
A Modular Vector Toolkit with a Tailored Set of Thermosensors To Regulate Gene Expression in Thermus thermophilus
Sign Up to like & getrecommendations! Published in 2019 at "ACS Omega"
DOI: 10.1021/acsomega.9b02107
Abstract: Modular plasmid architectures have shown to be a very useful resource to standardize, build, share, and compare biological parts and functional vectors, and are being applied in an increasing number of microorganisms. Here, we present… read more here.
Keywords: expression thermus; thermus; toolkit; expression ... See more keywords
Cryo-EM structure of the hibernating Thermus thermophilus 100S ribosome reveals a protein-mediated dimerization mechanism
Sign Up to like & getrecommendations! Published in 2018 at "Nature Communications"
DOI: 10.1038/s41467-018-06724-x
Abstract: In response to cellular stresses bacteria conserve energy by dimerization of ribosomes into inactive hibernating 100S ribosome particles. Ribosome dimerization in Thermus thermophilus is facilitated by hibernation-promoting factor (TtHPF). In this study we demonstrate high… read more here.
Keywords: cryo structure; dimerization; thermophilus 100s; 100s ribosome ... See more keywords
Occurrence of randomly recombined functional 16S rRNA genes in Thermus thermophilus suggests genetic interoperability and promiscuity of bacterial 16S rRNAs
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-47807-z
Abstract: Based on the structural complexity of ribosomes, 16S rRNA genes are considered species-specific and hence used for bacterial phylogenetic analysis. However, a growing number of reports suggest the occurrence of horizontal gene transfer, raising genealogical… read more here.
Keywords: rrna; rrna genes; 16s rrna; thermus thermophilus ... See more keywords