Directed Evolution of Aerotolerance in Sulfide-Dependent Thiazole Synthases
Sign Up to like & getrecommendations! Published in 2022 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.2c00512
Abstract: Sulfide-dependent THI4 thiazole synthases could potentially be used to replace plant cysteine-dependent suicide THI4s, whose high protein turnover rates make thiamin synthesis exceptionally energy-expensive. However, sulfide-dependent THI4s are anaerobic or microoxic enzymes and hence unadapted… read more here.
Keywords: sulfide dependent; thi4s; thiazole synthases; directed evolution ... See more keywords
Structure and function of aerotolerant, multiple-turnover THI4 thiazole synthases
Sign Up to like & getrecommendations! Published in 2021 at "Biochemical Journal"
DOI: 10.1042/bcj20210565
Abstract: Plant and fungal THI4 thiazole synthases produce the thiamin thiazole moiety in aerobic conditions via a single-turnover suicide reaction that uses an active-site Cys residue as sulfur donor. Multiple-turnover (i.e. catalytic) THI4s lacking an active-site… read more here.
Keywords: turnover; thi4s; thi4 thiazole; cys ... See more keywords