Varying thin filament activation in the framework of the Huxley'57 model
Sign Up to like & getrecommendations! Published in 2022 at "International Journal for Numerical Methods in Biomedical Engineering"
DOI: 10.1002/cnm.3655
Abstract: Muscle contraction is triggered by the activation of the actin sites of the thin filament by calcium ions. It results that the thin filament activation level varies over time. Moreover, this activation process is also… read more here.
Keywords: thin filament; huxley; framework; model ... See more keywords
Structure and Dynamics of the Flexible Cardiac Troponin T Linker Domain in a Fully Reconstituted Thin Filament.
Sign Up to like & getrecommendations! Published in 2022 at "Biochemistry"
DOI: 10.1021/acs.biochem.2c00091
Abstract: The structural analysis of large protein complexes has been greatly enhanced through the application of electron microscopy techniques. One such multiprotein complex, the cardiac thin filament (cTF), has cyclic interactions with thick filament proteins to… read more here.
Keywords: thin filament; structure; linker; ctnt linker ... See more keywords
Skeletal myosin binding protein-C isoforms regulate thin filament activity in a Ca2+-dependent manner
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-21053-1
Abstract: Muscle contraction, which is initiated by Ca2+, results in precise sliding of myosin-based thick and actin-based thin filament contractile proteins. The interactions between myosin and actin are finely tuned by three isoforms of myosin binding… read more here.
Keywords: myosin binding; muscle; thin filament; binding protein ... See more keywords
CAP2 is a regulator of actin pointed end dynamics and myofibrillogenesis in cardiac muscle
Sign Up to like & getrecommendations! Published in 2021 at "Communications Biology"
DOI: 10.1038/s42003-021-01893-w
Abstract: The precise assembly of actin-based thin filaments is crucial for muscle contraction. Dysregulation of actin dynamics at thin filament pointed ends results in skeletal and cardiac myopathies. Here, we discovered adenylyl cyclase-associated protein 2 (CAP2)… read more here.
Keywords: actin; muscle; cardiac muscle; thin filaments ... See more keywords
Revealing the mechanism of how cardiac myosin-binding protein C N-terminal fragments sensitize thin filaments for myosin binding
Sign Up to like & getrecommendations! Published in 2019 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.1816480116
Abstract: Significance Diverse demands on cardiac muscle require the fine-tuning of contraction. Cardiac myosin binding protein-C (cMyBP-C) is involved in this regulation; however, its precise molecular mechanism of action remains uncertain. By imaging the interactions of… read more here.
Keywords: calcium; thin filament; myosin binding; mechanism ... See more keywords
Structures from intact myofibrils reveal mechanism of thin filament regulation through nebulin
Sign Up to like & getrecommendations! Published in 2022 at "Science"
DOI: 10.1126/science.abn1934
Abstract: In skeletal muscle, nebulin stabilizes and regulates the length of thin filaments, but the underlying mechanism remains nebulous. In this work, we used cryo–electron tomography and subtomogram averaging to reveal structures of native nebulin bound… read more here.
Keywords: thin filament; muscle; thin filaments; myosin ... See more keywords
Abstract 339: Biomechanics and Calcium Handling of Thin Filament Hypertrophic Cardiomyopathy Variants
Sign Up to like & getrecommendations! Published in 2019 at "Circulation Research"
DOI: 10.1161/res.125.suppl_1.339
Abstract: The mechanism by which genetic variants of the thin filament cause hypertrophic cardiomyopathy (HCM) has not been fully elucidated. Induced pluripotent stem cell (iPSC)-derived cardiomyocytes can provide a rapidly generatable disease modeling tool to study… read more here.
Keywords: biomechanics; calcium handling; hypertrophic cardiomyopathy; thin filament ... See more keywords
Modeling Human Cardiac Thin Filament Structures
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Physiology"
DOI: 10.3389/fphys.2022.932333
Abstract: Striated muscle contraction is regulated in a calcium-dependent manner through dynamic motions of the tropomyosin/troponin polymer, a multicomponent complex wrapped around actin-containing thin filaments. Tropomyosin/troponin sterically blocks myosin-binding at low-calcium concentrations but moves to expose… read more here.
Keywords: thin filament; calcium; modeling human; tropomyosin troponin ... See more keywords
The Mechanisms of Thin Filament Assembly and Length Regulation in Muscles
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms23105306
Abstract: The actin containing tropomyosin and troponin decorated thin filaments form one of the crucial components of the contractile apparatus in muscles. The thin filaments are organized into densely packed lattices interdigitated with myosin-based thick filaments.… read more here.
Keywords: mechanisms thin; thin filament; assembly length; thin filaments ... See more keywords
Molecular Dynamics Assessment of Mechanical Properties of the Thin Filaments in Cardiac Muscle
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24054792
Abstract: Contraction of cardiac muscle is regulated by Ca2+ ions via regulatory proteins, troponin (Tn), and tropomyosin (Tpm) associated with the thin (actin) filaments in myocardial sarcomeres. The binding of Ca2+ to a Tn subunit causes… read more here.
Keywords: thin filament; molecular dynamics; muscle; cardiac muscle ... See more keywords