New Thiol-Reactive Eu-Complex for Distance Measurements by LRET
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2016.11.2425
Abstract: Crystallography and NMR spectroscopy are ideally suited to resolve the 3D structures of biomolecules but the material and time demand for each structure is high. Fluorescence resonance energy transfer (FRET) provides less structural information but… read more here.
Keywords: energy transfer; distance measurements; reactive complex; distance ... See more keywords
Redox active or thiol reactive? Optimization of rapid screens to identify less evident nuisance compounds.
Sign Up to like & getrecommendations! Published in 2022 at "Drug discovery today"
DOI: 10.1016/j.drudis.2022.03.008
Abstract: Compounds that exhibit assay interference or undesirable mechanisms of bioactivity are routinely encountered in assays at various stages of drug discovery. We observed that assays for the investigation of thiol-reactive and redox-active compounds have not… read more here.
Keywords: active thiol; thiol reactive; nuisance compounds; redox active ... See more keywords
Thiol Reactome: A Nontargeted Strategy to Precisely Identify Thiol Reactive Drinking Water Disinfection Byproducts.
Sign Up to like & getrecommendations! Published in 2023 at "Environmental science & technology"
DOI: 10.1021/acs.est.2c05486
Abstract: The precise identification of predominant toxic disinfection byproducts (DBPs) from disinfected water is a longstanding challenge. We propose a new acellular analytical strategy, the 'Thiol Reactome', to identify thiol-reactive DBPs by employing a thiol probe… read more here.
Keywords: gsh; disinfection byproducts; thiol reactome; water ... See more keywords
Conjugation of Peptides to Thiol-Reactive Gel for Affinity Purification of Antibodies.
Sign Up to like & getrecommendations! Published in 2019 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot099168
Abstract: Thiol-reactive linkers, such as iodoacetyl or maleimide, bound to cross-linked agarose are used to attach cysteine-containing peptides covalently to this resin for use in affinity-purification protocols. It is critical to confirm that the peptide contains… read more here.
Keywords: conjugation peptides; affinity purification; thiol reactive; peptides thiol ... See more keywords