Development of a fluorescent three‐hybrid system for the identification of protein‐protein associators
Sign Up to like & getrecommendations! Published in 2020 at "Peptide Science"
DOI: 10.1002/pep2.24178
Abstract: The chemically‐induced dimerization of proteins is fundamental to many key regulatory pathways. A number of natural products exert their downstream effect through the stabilization of a protein complex, either by direct binding to two distinct… read more here.
Keywords: protein; hybrid system; system; three hybrid ... See more keywords
The Yeast Three-Hybrid System for Protein Interactions.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7871-7_12
Abstract: Proteins rarely act alone as their functions tend to be regulated in vivo. Therefore, protein-protein interaction analyses provide key clues for understanding the complex biological processes in the living cell. Several techniques have been developed… read more here.
Keywords: protein; hybrid system; three hybrid; protein interactions ... See more keywords
The Yeast Three-Hybrid System for Screening RNA-Binding Proteins in Plants.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7871-7_13
Abstract: Yeast-hybrid methods have been successfully applied for screening interacting partners of DNAs or proteins. A yeast-based method, the yeast three-hybrid system, using a chimeric protein of a DNA-binding domain (LexA or GAL4BD) with a protein… read more here.
Keywords: hybrid system; three hybrid; binding proteins; rna binding ... See more keywords
The three-hybrid genetic composition of an Ecuadorian population using AIMs-InDels compared with autosomes, mitochondrial DNA and Y chromosome data
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-45723-w
Abstract: The history of Ecuador was marked by the arrival of Europeans with Africans, resulting in the mixture of Native Americans with Africans and Europeans. The present study contributes to the knowledge of the Ecuadorian mestizo… read more here.
Keywords: hybrid genetic; genetic composition; three hybrid; mitochondrial dna ... See more keywords
Optimization of a bacterial three-hybrid assay through in vivo titration of an RNA-DNA adapter-protein.
Sign Up to like & getrecommendations! Published in 2021 at "RNA"
DOI: 10.1261/rna.077404.120
Abstract: Non-coding RNAs regulate gene expression in every domain of life. In bacteria, small RNAs (sRNAs) regulate gene expression in response to stress and are often assisted by RNA-chaperone proteins, such as Hfq. We have recently… read more here.
Keywords: protein; dna adapter; three hybrid; rna dna ... See more keywords
Rapid and predictable genome evolution across three hybrid ant populations
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DOI: 10.1371/journal.pbio.3001914
Abstract: Hybridization is frequent in the wild but it is unclear whether admixture events lead to predictable outcomes and if so, at what timescale. We show that selection led to correlated sorting of genetic variation in… read more here.
Keywords: ant populations; predictable genome; three hybrid; rapid predictable ... See more keywords
Using the yeast three-hybrid system for the identification of small molecule-protein interactions with the example of ethinylestradiol
Sign Up to like & getrecommendations! Published in 2022 at "Biological Chemistry"
DOI: 10.1515/hsz-2021-0355
Abstract: Abstract Since the first report on a yeast three-hybrid system, several approaches have successfully utilized different setups for discovering targets of small molecule drugs. Compared to broadly applied MS based target identification approaches, the yeast… read more here.
Keywords: system; three hybrid; identification; yeast three ... See more keywords