Improving Structural Stability and Anticoagulant Activity of a Thrombin Binding Aptamer by Aromatic Modifications
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202100670
Abstract: The thrombin binding aptamer (TBA) is a 15‐mer DNA oligonucleotide (5′‐GGT TGG TGT GGT TGG‐3′), that can form a stable intramolecular antiparallel chair‐like G‐quadruplex structure. This aptamer shows anticoagulant properties by interacting with one of… read more here.
Keywords: thrombin binding; stability; anticoagulant activity; binding aptamer ... See more keywords
Structural and mechanistic insights into modified G-quadruplex thrombin-binding DNA aptamers.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2019.04.025
Abstract: Thrombin-binding aptamer (TBA) can fold into a G-quadruplex structure necessary for interacting with thrombin. When one thymidine residue of the TGT loop at position 7 is replaced with unlocked uracil (UNA), d-isothymidine (D-isoT) or l-isothymidine… read more here.
Keywords: structural mechanistic; isot una; thrombin; insights modified ... See more keywords
The "Janus face" of the thrombin binding aptamer: Investigating the anticoagulant and antiproliferative properties through straightforward chemical modifications.
Sign Up to like & getrecommendations! Published in 2018 at "Bioorganic chemistry"
DOI: 10.1016/j.bioorg.2017.11.005
Abstract: BACKGROUND The thrombin binding aptamer (TBA) is endowed with both anticoagulant and antiproliferative activities. Its chemico-physical and/or biological properties can be tuned by the site-specific replacement of selected residues. METHODS Four oligodeoxynucleotides (ODNs) based on… read more here.
Keywords: binding aptamer; anticoagulant antiproliferative; antiproliferative properties; residues positions ... See more keywords
Methoxymethyl Threofuranosyl Thymidine (4′-MOM-TNA-T) at the T7 Position of the Thrombin-Binding Aptamer Boosts Anticoagulation Activity, Thermal Stability, and Nuclease Resistance
Sign Up to like & getrecommendations! Published in 2020 at "ACS Omega"
DOI: 10.1021/acsomega.9b03042
Abstract: The synthesis of 4′-methoxymethyl threofuranosyl (4′-MOM-TNA) thymidine and derived oligomers of the G-rich thrombin-binding aptameric (TBA) sequence is reported. The G-quadruplex stability, anticoagulation activity, and the enzymatic stability of these oligomers bearing the 2′-3′-phosphodiester backbone… read more here.
Keywords: methoxymethyl threofuranosyl; thrombin binding; mom tna; stability ... See more keywords
Unlike RNA-TBA (rTBA), iso-rTBA, the 2'-5'-linked RNA-thrombin-binding aptamer, is a functional equivalent of TBA.
Sign Up to like & getrecommendations! Published in 2023 at "Chemical communications"
DOI: 10.1039/d2cc05718b
Abstract: An antiparallel, functional RNA G-quadruplex of the 2'-5'-linked thrombin-binding aptamer (iso-rTBA) is reported for the first time. It can inhibit clotting and is remarkably stable to nuclease-degradation, besides having high thermal stability. It is thus,… read more here.
Keywords: tba; binding aptamer; tba rtba; rtba ... See more keywords
Binding mode of a cationic porphyrin to parallel and antiparallel thrombin binding aptamer G-quadruplex
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Biomolecular Structure and Dynamics"
DOI: 10.1080/07391102.2019.1642241
Abstract: Abstract The spectral properties of meso-tetrakis (N-methylpyridinium-4-yl)porphyrin (TMPyP) in the presence of parallel and antiparallel G-quadruplexes formed from a thrombin-binding aptamer G-quadruplex (5′-G3T2G3TGTG3T2G3) were investigated in this study. Red shift and hypochromism in the Soret… read more here.
Keywords: binding aptamer; antiparallel quadruplexes; porphyrin; parallel antiparallel ... See more keywords
Structural properties and anticoagulant/cytotoxic activities of heterochiral enantiomeric thrombin binding aptamer (TBA) derivatives
Sign Up to like & getrecommendations! Published in 2020 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkaa1109
Abstract: Abstract The thrombin binding aptamer (TBA) possesses promising antiproliferative properties. However, its development as an anticancer agent is drastically impaired by its concomitant anticoagulant activity. Therefore, suitable chemical modifications in the TBA sequence would be… read more here.
Keywords: aptamer tba; tba; tba derivatives; binding aptamer ... See more keywords
In silico direct folding of thrombin-binding aptamer G-quadruplex at all-atom level
Sign Up to like & getrecommendations! Published in 2017 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkx1079
Abstract: Abstract The reversible folding of the thrombin-binding DNA aptamer G-quadruplexes (GQs) (TBA-15) starting from fully unfolded states was demonstrated using a prolonged time scale (10–12 μs) parallel tempering metadynamics (PTMetaD) simulation method in conjunction with… read more here.
Keywords: energy; direct folding; folding thrombin; thrombin binding ... See more keywords
A case of thrombomodulin mutation causing defective thrombin binding with absence of protein C and TAFI activation.
Sign Up to like & getrecommendations! Published in 2020 at "Blood advances"
DOI: 10.1182/bloodadvances.2019001155
Abstract: Thrombomodulin functions as an anticoagulant through thrombin binding and protein C activation. We herein report the first case of hereditary functional thrombomodulin deficiency presenting with recurrent subcutaneous hemorrhage and old cerebral infarction. The patient had… read more here.
Keywords: protein; thrombomodulin mutation; case; activation ... See more keywords
Dual Aptamer-Functionalized 3D Plasmonic Metamolecule for Thrombin Sensing
Sign Up to like & getrecommendations! Published in 2019 at "Applied Sciences"
DOI: 10.3390/app9153006
Abstract: DNA nanotechnology offers the possibility to rationally design structures with emergent properties by precisely controlling their geometry and functionality. Here, we demonstrate a DNA-based plasmonic metamolecule that is capable of sensing human thrombin proteins. The… read more here.
Keywords: thrombin; metamolecule; plasmonic metamolecule; dual aptamer ... See more keywords
Thrombin-Binding Aptamer with Inversion of Polarity Sites (IPS): Effect on DNAzyme Activity and Anticoagulant Properties
Sign Up to like & getrecommendations! Published in 2021 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms22157902
Abstract: In this work we examined the properties of thrombin-binding aptamer (TBA) modified by the introduction of inversion of polarity sites (IPS) in order to assess the effect of modification on the activation of TBA to… read more here.
Keywords: inversion; dnazyme; inversion polarity; activity ... See more keywords