High-Throughput Assay for RhoGEFs Based on the Transcreener® GDP Assay.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8612-5_12
Abstract: We describe a high-throughput screening (HTS)-compatible method for detecting GTPase exchange factor (GEF) activity based on stimulation of GDP formation by Rho GTPases. The method is based on the fact that GDP dissociation is the… read more here.
Keywords: throughput assay; transcreener gdp; gdp formation; high throughput ... See more keywords
A High-Throughput Assay to Identify Allosteric Inhibitors of the PLC-γ Isozymes Operating at Membranes.
Sign Up to like & getrecommendations! Published in 2020 at "Biochemistry"
DOI: 10.1021/acs.biochem.0c00511
Abstract: The two phospholipase C-γ (PLC-γ) isozymes are major signaling hubs and emerging therapeutic targets for various diseases, yet there are no selective inhibitors for these enzymes. We have developed a high-throughput, liposome-based assay that features… read more here.
Keywords: assay identify; plc isozymes; allosteric inhibitors; throughput assay ... See more keywords