Overview of High-Throughput Cloning Methods for the Post-genomic Era.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9624-7_1
Abstract: The advent of new DNA sequencing technologies leads to a dramatic increase in the number of available genome sequences and therefore of target genes with potential for functional analysis. The insertion of these sequences into… read more here.
Keywords: throughput; cloning methods; methods post; throughput cloning ... See more keywords
An Optimised CRISPR/Cas9 Adenovirus vector (AdZ-CRISPR) for high throughput cloning of sgRNA, using enhanced sgRNA and Cas9 variants.
Sign Up to like & getrecommendations! Published in 2022 at "Human gene therapy"
DOI: 10.1089/hum.2021.120
Abstract: Recombinant Adenovirus vectors enable highly efficient gene delivery in vitro and in vivo. As a result, they are widely used in gene therapy, vaccination, and anti-cancer applications. We have previously developed the AdZ vector system,… read more here.
Keywords: crispr cas9; cas9; high throughput; vector ... See more keywords