High-Throughput Enzyme Kinetics with 3D Microfluidics and Imaging SAMDI Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.8b04391
Abstract: Microfluidic systems are important for performing precise reagent manipulations and reducing material consumption in biological assays. However, optical detection methods limit analyses to fluorescent or UV-active compounds and traditional 2D fluidic designs have limited degrees… read more here.
Keywords: throughput enzyme; mass; mass spectrometry; high throughput ... See more keywords
Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments
Sign Up to like & getrecommendations! Published in 2023 at "Chemical Reviews"
DOI: 10.1021/acs.chemrev.2c00910
Abstract: Novel and improved biocatalysts are increasingly sourced from libraries via experimental screening. The success of such campaigns is crucially dependent on the number of candidates tested. Water-in-oil emulsion droplets can replace the classical test tube,… read more here.
Keywords: vitro compartments; droplet; ultrahigh throughput; droplet screening ... See more keywords