High-throughput functional characterization of protein phosphorylation sites in yeast.
Sign Up to like & getrecommendations! Published in 2021 at "Nature biotechnology"
DOI: 10.1038/s41587-021-01051-x
Abstract: Phosphorylation is a critical post-translational modification involved in the regulation of almost all cellular processes. However, fewer than 5% of thousands of recently discovered phosphosites have been functionally annotated. In this study, we devised a… read more here.
Keywords: functional characterization; throughput functional; characterization protein; phosphorylation ... See more keywords
SorTn-seq: a high-throughput functional genomics approach to discovering regulators of bacterial gene expression.
Sign Up to like & getrecommendations! Published in 2021 at "Nature protocols"
DOI: 10.1038/s41596-021-00582-6
Abstract: We recently developed a high-throughput functional genomics approach, named 'SorTn-seq', to identify factors affecting expression of any gene of interest in bacteria. Our approach facilitates high-throughput screening of complex mutant pools, a task previously hindered… read more here.
Keywords: functional genomics; throughput functional; expression; sortn seq ... See more keywords