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Published in 2021 at "Nature biotechnology"
DOI: 10.1038/s41587-021-01051-x
Abstract: Phosphorylation is a critical post-translational modification involved in the regulation of almost all cellular processes. However, fewer than 5% of thousands of recently discovered phosphosites have been functionally annotated. In this study, we devised a…
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Keywords:
functional characterization;
throughput functional;
characterization protein;
phosphorylation ... See more keywords
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Published in 2021 at "Nature protocols"
DOI: 10.1038/s41596-021-00582-6
Abstract: We recently developed a high-throughput functional genomics approach, named 'SorTn-seq', to identify factors affecting expression of any gene of interest in bacteria. Our approach facilitates high-throughput screening of complex mutant pools, a task previously hindered…
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Keywords:
functional genomics;
throughput functional;
expression;
sortn seq ... See more keywords