Quantification of Intracellular Accumulation and Retention of Lysosomotropic Macrocyclic Compounds by High-Throughput Imaging of Lysosomal Changes.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of pharmaceutical sciences"
DOI: 10.1016/j.xphs.2018.11.001
Abstract: Many marketed pharmaceuticals reach extremely high tissue concentrations due to accumulation in lysosomes (lysosomotropism). Quantitative prediction of intracellular concentrations of accumulating drugs is challenging, especially for macrocyclic compounds that mainly do not fit in current… read more here.
Keywords: macrocyclic compounds; intracellular accumulation; accumulation; throughput imaging ... See more keywords
HiHiMap: single-cell quantitation of histones and histone posttranslational modifications across the cell cycle by high-throughput imaging
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Biology of the Cell"
DOI: 10.1091/mbc.e16-12-0870
Abstract: High-throughput Histone Mapping (HiHiMap) is an automated high-throughput imaging technique to determine histone and histone PTMs across the cell cycle at the single-cell level in a highly parallel format. The method is widely applicable to… read more here.
Keywords: across cell; cell cycle; throughput imaging; cell ... See more keywords
High-throughput imaging and quantitative analysis uncovers the nature of plasmid positioning by ParABS
Sign Up to like & getrecommendations! Published in 2022 at "eLife"
DOI: 10.7554/elife.78743
Abstract: The faithful segregation and inheritance of bacterial chromosomes and low-copy number plasmids requires dedicated partitioning systems. The most common of these, ParABS, consists of ParA, a DNA-binding ATPase and ParB a protein that binds to… read more here.
Keywords: nature plasmid; positioning parabs; throughput imaging; high throughput ... See more keywords