Optimizing depth and type of high‐throughput sequencing data for microsatellite discovery
Sign Up to like & getrecommendations! Published in 2019 at "Applications in Plant Sciences"
DOI: 10.1002/aps3.11298
Abstract: Premise Simple sequence repeat (SSR) markers (microsatellites) are a mainstay of many labs, especially when working on a limited budget, carrying out preliminary analyses, and in teaching. Whether SSRs mined from plant genomes or transcriptomes… read more here.
Keywords: optimizing depth; sequencing data; discovery; throughput sequencing ... See more keywords
Multiplex targeted high‐throughput sequencing in a series of 352 patients with congenital limb malformations
Sign Up to like & getrecommendations! Published in 2019 at "Human Mutation"
DOI: 10.1002/humu.23912
Abstract: Congenital limb malformations (CLM) comprise many conditions affecting limbs and more than 150 associated genes have been reported. Due to this large heterogeneity, a high proportion of patients remains without a molecular diagnosis. In the… read more here.
Keywords: limb malformations; congenital limb; sequencing series; throughput sequencing ... See more keywords
Application of high‐throughput sequencing for hereditary thrombocytopenia in southwestern China
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Clinical Laboratory Analysis"
DOI: 10.1002/jcla.23896
Abstract: The aim of this study was to design and analyze the applicability of a 21‐gene high‐throughput sequencing (HTS) panel in the molecular diagnosis of patients with hereditary thrombocytopenia (HT). read more here.
Keywords: sequencing hereditary; application high; hereditary thrombocytopenia; throughput sequencing ... See more keywords
Comparison of DNA purification methods for high‐throughput sequencing of fungal communities from wine fermentation
Sign Up to like & getrecommendations! Published in 2022 at "MicrobiologyOpen"
DOI: 10.1002/mbo3.1321
Abstract: High‐throughput sequencing approaches, which target a taxonomically discriminant locus, allow for in‐depth insight into microbial communities’ compositions. Although microorganisms are historically investigated by cultivation on artificial culture media, this method presents strong limitations, since only… read more here.
Keywords: dna; fungal communities; high throughput; sequencing fungal ... See more keywords
Universal Library Preparation Protocol for Efficient High-Throughput Sequencing of Double-Stranded RNA Viruses.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0138-9_14
Abstract: This chapter reports a library preparation protocol for efficient high-throughput sequencing of double-stranded RNA viruses. The protocol consists of four main steps, viz., enzyme treatment, precipitation using lithium chloride, full-length amplification of cDNAs, and tailing… read more here.
Keywords: rna viruses; double stranded; stranded rna; throughput sequencing ... See more keywords
Improved Analysis of High-Throughput Sequencing Data Using Small Universal k-Mer Hitting Sets.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1103-6_5
Abstract: High-throughput sequencing machines can read millions of DNA molecules in parallel in a short time and at a relatively low cost. As a consequence, researchers have access to databases with millions of genomic samples. Searching… read more here.
Keywords: universal hitting; hitting sets; throughput sequencing; using small ... See more keywords
Viral Double-Stranded RNAs (dsRNAs) from Plants: Alternative Nucleic Acid Substrates for High-Throughput Sequencing.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7683-6_4
Abstract: High-throughput sequencing (or next-generation sequencing-NGS) is an emerging technology that allows the detection of plant viruses without any prior knowledge. Various sequencing techniques and various templates can be used as substrate for NGS. This chapter… read more here.
Keywords: double stranded; stranded rnas; rnas dsrnas; throughput sequencing ... See more keywords
Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-Seq): Tips and Tricks Regarding the Laboratory Protocol and Initial Downstream Data Analysis.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7774-1_15
Abstract: Chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq) has become an essential tool for epigenetic scientists. ChIP-seq is used to map protein-DNA interactions and epigenetic marks such as histone modifications at the genome-wide level. Here we… read more here.
Keywords: chip seq; chip; sequencing chip; throughput sequencing ... See more keywords
Using High-Throughput Sequencing to Characterize the Development of the Antibody Repertoire During Infections: A Case Study of HIV-1.
Sign Up to like & getrecommendations! Published in 2017 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-3-319-72077-7_12
Abstract: High throughput sequencing (HTS) approaches have only recently been applied to describing the antibody/B-cell repertoire in fine detail, but these data sets have already become critical to the design of vaccines and therapeutics, and monitoring… read more here.
Keywords: case study; antibody; repertoire; throughput sequencing ... See more keywords
Identification of nucleic acid aptamers against lactate dehydrogenase via SELEX and high-throughput sequencing
Sign Up to like & getrecommendations! Published in 2021 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-021-03397-2
Abstract: Nucleic acid aptamers are small fragments of DNA or RNA molecules binding specifically to targets, which can be obtained through in vitro screening via systematic evolution of ligands by exponential enrichment (SELEX). Lactate dehydrogenase (LDH)… read more here.
Keywords: throughput sequencing; ldh; lactate dehydrogenase; nucleic acid ... See more keywords
High-Throughput Sequencing of the 16S rRNA Gene as a Survey to Analyze the Microbiomes of Free-Living Ciliates Paramecium
Sign Up to like & getrecommendations! Published in 2019 at "Microbial Ecology"
DOI: 10.1007/s00248-019-01321-x
Abstract: Ciliates are the largest group of ubiquitous aquatic bacterivorous protists, and many species are easily cultivated. However, only few studies reported prokaryotic communities naturally associated with ciliate cells. Herein, we analyzed the microbiome composition of… read more here.
Keywords: 16s rrna; throughput sequencing; rrna gene; high throughput ... See more keywords