Rapid tilt-series acquisition for electron cryotomography.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of structural biology"
DOI: 10.1016/j.jsb.2018.12.008
Abstract: Using a new Titan Krios stage equipped with a single-axis holder, we developed two methods to accelerate the collection of tilt-series. We demonstrate a continuous-tilting method that can record a tilt-series in seconds, but with… read more here.
Keywords: rapid tilt; electron cryotomography; series; biology ... See more keywords
Characterization of micelle-templated silica nanotubes and nanotube bundles using tilt-series transmission electron microscopy
Sign Up to like & getrecommendations! Published in 2020 at "Microporous and Mesoporous Materials"
DOI: 10.1016/j.micromeso.2019.109760
Abstract: Abstract Pluronic-surfactant-templated silica and organosilica nanotubes, silica nanotube bundles and silicas with small ordered domains of cylindrical mesopores were characterized by using tilt-series transmission electron microscopy (TEM). In as-synthesized nanotube samples, three-dimensional (3-D) arrangements of… read more here.
Keywords: microscopy; silica nanotubes; nanotube bundles; templated silica ... See more keywords
Rapid Tilt-series Methods: The Future of Cryo-ET
Sign Up to like & getrecommendations! Published in 2020 at "Microscopy and Microanalysis"
DOI: 10.1017/s1431927620023491
Abstract: Cryo-electron tomography (Cryo-ET) has become an indispensable imaging technique to reveal the structures of biological complexes and the architectures of cells in a nearly native frozen-hydrated state. In Cryo-ET, a tilt-series is collected by tilting… read more here.
Keywords: cryo; rapid tilt; tilt series; methods future ... See more keywords
Volume Imaging By Tracking Sparse Topological Features In Electron Micrograph Tilt Series
Sign Up to like & getrecommendations! Published in 2022 at "Microscopy and Microanalysis"
DOI: 10.1017/s1431927622001805
Abstract: The sensitive coherent interference of electron waves arising from a specimen is useful for revealing subtle structural information in electron micrographs, which can be important for minimising dose and for rapid imaging. In general, dynamical… read more here.
Keywords: volume imaging; topological features; imaging tracking; tracking sparse ... See more keywords
GPU Accelerated 3D Tomographic Reconstruction and Visualization from Noisy Electron Microscopy Tilt-Series.
Sign Up to like & getrecommendations! Published in 2022 at "IEEE transactions on visualization and computer graphics"
DOI: 10.1109/tvcg.2022.3230445
Abstract: We present a novel framework for 3D tomographic reconstruction and visualization of tomograms from noisy electron microscopy tilt-series. Our technique takes as an input aligned tilt-series from cryogenic electron microscopy and creates denoised 3D tomograms… read more here.
Keywords: visualization; electron microscopy; microscopy; reconstruction ... See more keywords
Transmission Electron Microscopy Tilt-Series Data from In-Situ Chondrocyte Primary Cilia
Sign Up to like & getrecommendations! Published in 2021 at "Data"
DOI: 10.3390/data6110118
Abstract: The primary cilium has recently become the focus of intensive investigations into understanding the physical structure and processes of eukaryotic cells. This paper describes two tilt-series image datasets, acquired by transmission electron microscopy, of in… read more here.
Keywords: tilt series; electron microscopy; microscopy; primary cilia ... See more keywords