Evaluation of the performance of two real‐time PCR assays for detecting Mycobacterium species
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Clinical Laboratory Analysis"
DOI: 10.1002/jcla.22645
Abstract: Rapid discrimination between Mycobacterium tuberculosis (MTB) and nontuberculous mycobacteria (NTM) is critical for patient treatment and to avoid unnecessary expenditure on infection control. Because real‐time PCR assays distinguish MTB from NTM, we evaluated the performance… read more here.
Keywords: time pcr; pcr assays; performance two; real time ... See more keywords
Establishment of an in‐house real‐time RT‐PCR assay for the detection of severe acute respiratory syndrome coronavirus 2 using the first World Health Organization international standard in a resource‐limited country
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Clinical Laboratory Analysis"
DOI: 10.1002/jcla.24355
Abstract: The COVID‐19 pandemic caused by SARS‐CoV‐2 remains public health burdens and many unresolved issues worldwide. Molecular assays based on real‐time RT‐PCR are critical for the detection of SARS‐CoV‐2 in clinical specimens from patients suspected of… read more here.
Keywords: health; time pcr; detection; real time ... See more keywords
Development of molecular markers to distinguish between morphologically similar edible plants and poisonous plants using real-time PCR assay.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of the science of food and agriculture"
DOI: 10.1002/jsfa.10711
Abstract: BACKGROUND Due to a similar appearance between edible and poisonous plants, a total of 42 patients ingesting poisonous plants have occurred from 2013 to 2017 in Korea. We developed species-specific primer sets of three of… read more here.
Keywords: real time; time pcr; primer sets; plants using ... See more keywords
Development of conventional PCR and real‐time PCR assays to discriminate the origins of Chinese pepper oil and herbal materials from Zanthoxylum
Sign Up to like & getrecommendations! Published in 2019 at "Journal of the Science of Food and Agriculture"
DOI: 10.1002/jsfa.9458
Abstract: Abstract BACKGROUND To ensure the safety, quality and therapeutic efficacy of processed foods and herbal medicines, it is important to identify and discriminate economically motivated adulterants. Zanthoxylum schinifolium is sold at a higher price than… read more here.
Keywords: medicine; real time; time pcr; pcr ... See more keywords
A real-time PCR for detection of pathogens of anthracnose on Chinese fir using TaqMan probe targeting ApMat gene.
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DOI: 10.1002/ps.7260
Abstract: BACKGROUND Anthracnose is one of the most widespread and destructive diseases on Chinese fir. Colletotrichum cangyuanense, C. fructicola, C. gloeosporioides, and C. siamense are the causal agents of anthracnose on Chinese fir. A rapid and… read more here.
Keywords: gloeosporioides siamense; time pcr; fructicola gloeosporioides; real time ... See more keywords
DNA Extraction from Clinical Specimens for the Direct Detection of Mycobacterium ulcerans by Real-Time PCR.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1779-3_6
Abstract: Mycobacterium ulcerans is a slow-growing environmental bacterium that causes a severe skin disease known as Buruli ulcer (BU). Rapid detection of M. ulcerans in clinical specimens is essential for early diagnosis so that patients can… read more here.
Keywords: mycobacterium ulcerans; time pcr; detection; real time ... See more keywords
Detection of Mycobacterium ulcerans DNA Using Real-Time PCR.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1779-3_8
Abstract: Mycobacterium ulcerans is a slow-growing environmental bacterium that causes a severe skin disease known as Buruli ulcer. Rapid detection of M. ulcerans in clinical specimens is essential for ensuring early diagnosis and prevention of disability.… read more here.
Keywords: mycobacterium ulcerans; detection; real time; time pcr ... See more keywords
Detection of Food Allergens by Taqman Real-Time PCR Methodology.
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DOI: 10.1007/978-1-4939-6925-8_8
Abstract: Real-time PCR (polymerase chain reaction) has shown to be a very effective technology for the detection of food allergens. The protocol described herein consists on a real-time PCR assay targeting the plant ITS (Internal Transcribed… read more here.
Keywords: methodology; detection; real time; time pcr ... See more keywords
Fast Real-Time PCR for the Detection of Crustacean Allergens in Foods.
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DOI: 10.1007/978-1-4939-7060-5_11
Abstract: Crustaceans are one of the most common allergens causing severe food reaction. Hypersensitivity reactions associated with seafood intake are one of the most common food allergies in adults. Crustaceans including shrimps, prawns, crabs, lobster, and… read more here.
Keywords: detection; real time; time pcr; fast real ... See more keywords
Using Quantitative Real-Time PCR to Detect MicroRNA Expression Profile During Embryonic Stem Cell Differentiation.
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DOI: 10.1007/978-1-4939-7108-4_18
Abstract: Quantitative real-time PCR (qRT-PCR) is a reliable method to determine and monitor microRNA (miRNA) expression profiles in different cells, tissues, and organisms. Although there are several different strategies in performing qRT-PCR to determine miRNA expression,… read more here.
Keywords: real time; quantitative real; time pcr; pcr ... See more keywords
Multiplex Real-Time PCR Using Encoded Microparticles for MicroRNA Profiling.
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DOI: 10.1007/978-1-4939-7231-9_15
Abstract: Multiplex quantitative real-time PCR (qPCR), which measures multiple DNAs in a given sample, has drawn unprecedented attention as a means of verifying the rapidly increasing genetic targets in a single phenotype. We report the detailed… read more here.
Keywords: multiplex real; real time; time pcr; pcr ... See more keywords