Articles with "time polymerase" as a keyword



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Investigation of BK Virus by Real-Time Polymerase Chain Reaction in Patients With Allogeneic Bone Marrow Transplantation.

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Published in 2018 at "Transplantation proceedings"

DOI: 10.1016/j.transproceed.2017.12.056

Abstract: INTRODUCTION BK virus (BKV) is a common human polyomavirus and causes latent infection. Especially in immunosuppressive patients, early diagnosis and treatment are very important in reducing the high mortality rate. In this study, we investigated… read more here.

Keywords: real time; bkv; time polymerase; transplantation ... See more keywords
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Machine Learning-Assisted Real-Time Polymerase Chain Reaction and High-Resolution Melt Analysis for SARS-CoV-2 Variant Identification

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Published in 2023 at "Analytical Chemistry"

DOI: 10.1021/acs.analchem.2c05112

Abstract: Since the declaration of COVID-19 as a pandemic in early 2020, multiple variants of the severe acute respiratory syndrome-related coronavirus (SARS-CoV-2) have been detected. The emergence of multiple variants has raised concerns due to their… read more here.

Keywords: cov variant; variant identification; machine learning; time polymerase ... See more keywords
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Optimizing Primer and Probe Concentrations for Use in Real-Time Polymerase Chain Reaction (PCR) Assays.

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Published in 2018 at "Cold Spring Harbor protocols"

DOI: 10.1101/pdb.prot095018

Abstract: Once primers and probes have been designed and obtained, it is necessary to optimize their concentration for each real-time polymerase chain reaction (PCR) assay. A set of PCRs is assembled in which the concentrations of… read more here.

Keywords: real time; time polymerase; chain reaction; reaction pcr ... See more keywords
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Constructing a Standard Curve for Real-Time Polymerase Chain Reaction (PCR) Experiments.

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Published in 2018 at "Cold Spring Harbor protocols"

DOI: 10.1101/pdb.prot095026

Abstract: It is essential to prepare a standard curve for every real-time polymerase chain reaction (PCR) experiment. This protocol is used to construct a standard curve in which the template concentration is unknown. Such a standard… read more here.

Keywords: real time; time polymerase; curve; polymerase chain ... See more keywords
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Selection of reference genes for transcript profiling of Sargassum polycystum by quantitative real‐time polymerase chain reaction

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Published in 2018 at "Phycological Research"

DOI: 10.1111/pre.12328

Abstract: Sargassum species are one of the major alginate‐producing seaweed species in Asian countries. Alginate is widely used in food, feed, pharmaceutical and medical industries as thickening and stabilizing agents. To establish a set of consistently… read more here.

Keywords: real time; quantitative real; time polymerase; reference ... See more keywords
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Detection of SARS-CoV-2 using real-time polymerase chain reaction in different clinical specimens: A critical review.

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Published in 2021 at "Allergologia et immunopathologia"

DOI: 10.15586/aei.v49i1.60

Abstract: Coronavirus disease 2019 (COVID-19) is a disease caused by a new strain of coronavirus named as severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Globally, since the outbreak, more than seven million confirmed cases of COVID-19 have… read more here.

Keywords: real time; time; time polymerase; chain reaction ... See more keywords
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Study of Optimal Real-time Polymerase Chain Reaction Chip by Comparison of Coating Reflective Materials

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Published in 2019 at "Sensors and Materials"

DOI: 10.18494/sam.2019.2101

Abstract: The real-time polymerase chain reaction (RT-PCR) test chip employed in previous experiments used a matte black printed circuit board (PCB) substrate printed with a white silk legend on a heating pattern. However, when analyzing the… read more here.

Keywords: real time; time polymerase; chain reaction; polymerase chain ... See more keywords
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Clinical epidemiological applicability of real-time polymerase chain reaction for COVID-19

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Published in 2022 at "Osong Public Health and Research Perspectives"

DOI: 10.24171/j.phrp.2022.0135

Abstract: Objectives Real-time polymerase chain reaction is currently used as a confirmatory test for coronavirus disease 2019 (COVID-19). The test results are interpreted as positive, negative, or inconclusive, and are used only for a qualitative classification… read more here.

Keywords: polymerase; chain reaction; polymerase chain; time polymerase ... See more keywords
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Performance and Cost-effectiveness of Pooling COVID-19 Samples Using Real time Polymerase Chain Reaction Test.

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Published in 2022 at "Journal of Nepal Health Research Council"

DOI: 10.33314/jnhrc.v20i02.3974

Abstract: BACKGROUND Rapid detection of Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2) by real-time polymerase chain reaction (RT-PCR) is the most reliable method used worldwide. Although the incidence of the disease has increased globally, the… read more here.

Keywords: time; chain reaction; polymerase chain; cost ... See more keywords
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Real-Time Polymerase Chain Reaction: Current Techniques, Applications, and Role in COVID-19 Diagnosis

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Published in 2022 at "Genes"

DOI: 10.3390/genes13122387

Abstract: Successful detection of the first SARS-CoV-2 cases using the real-time polymerase chain reaction (real-time PCR) method reflects the power and usefulness of this technique. Real-time PCR is a variation of the PCR assay to allow… read more here.

Keywords: time; time pcr; time polymerase; detection ... See more keywords
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New Method for Differentiation of Granuloviruses (Betabaculoviruses) Based on Real-Time Polymerase Chain Reaction (Real-Time PCR)

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Published in 2019 at "Viruses"

DOI: 10.3390/v11020115

Abstract: Baculoviridae is a highly diverse family of rod-shaped viruses with double-stranded DNA. To date, almost 100 species have had their complete genomic sequences deposited in the GenBank database, a quarter of which comprises granuloviruses (GVs).… read more here.

Keywords: real time; time; method; time polymerase ... See more keywords