Amplification and Detection of Minuscule Amounts of Misfolded Prion Protein by Using the Real-Time Quaking-Induced Conversion.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7816-8_16
Abstract: A characteristic feature of transmissible spongiform encephalopathies (TSE) is the progressive accumulation of protein aggregates in the brain in a self-propagation manner. Based on this mechanism, in vitro protein amplification systems (such as real-time quaking-induced… read more here.
Keywords: protein; real time; prion; quaking induced ... See more keywords
Real-Time Quaking- Induced Conversion Assays for Prion Diseases, Synucleinopathies, and Tauopathies
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Aging Neuroscience"
DOI: 10.3389/fnagi.2022.853050
Abstract: Prion diseases, synucleinopathies and tauopathies are neurodegenerative disorders characterized by deposition of abnormal protein aggregates in brain and other tissues. These aggregates consist of misfolded forms of prion, α-synuclein (αSyn), or tau proteins that cause… read more here.
Keywords: prion; time quaking; prion diseases; synucleinopathies tauopathies ... See more keywords
Application of α-Syn Real-Time Quaking-Induced Conversion for Brain and Skin Specimens of the Chinese Patients With Parkinson’s Disease
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DOI: 10.3389/fnagi.2022.898516
Abstract: The real-time quaking-induced conversion (RT-QuIC) assay has been developed and used as an in vitro diagnostic tool for Parkinson’s disease (PD). In this study, we established α-Syn RT-QuIC using recombinant human α-Syn as the substrate.… read more here.
Keywords: time quaking; quic; quaking induced; brain ... See more keywords
Evaluation of the effect of various main elements on the PrPSc detection by real-time quaking-induced conversion assay
Sign Up to like & getrecommendations! Published in 2018 at "International Journal of Molecular Medicine"
DOI: 10.3892/ijmm.2018.3867
Abstract: In order to definitively diagnosis sporadic Creutzfeldt-Jakob disease (sCJD), brain tissue is currently required. Therefore, there is a great need for tests that can detect sCJD in body fluids or other types of tissues. Different… read more here.
Keywords: real time; quaking induced; induced conversion; prpsc detection ... See more keywords