A TIRF microscopy assay to decode how tau regulates EB's tracking at microtubule ends.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in cell biology"
DOI: 10.1016/bs.mcb.2017.06.013
Abstract: Tau is a major microtubule-associated protein (MAP) mainly expressed in the brain. Tau binds the lattice of microtubules and favors their elongation and bundling. Recent studies have shown that tau is also a partner of… read more here.
Keywords: microtubule ends; tau; microscopy; microtubule dynamics ... See more keywords
Light scattering in TIRF microscopy: a theoretical study of the limits to surface selectivity.
Sign Up to like & getrecommendations! Published in 2021 at "Biophysical journal"
DOI: 10.1016/j.bpj.2021.06.025
Abstract: In TIRF microscopy, the sample resides near a surface in an evanescent optical field that, ideally, decreases in intensity with distance from the surface in a pure exponential fashion. In practice, multiple surfaces and imperfections… read more here.
Keywords: surface selectivity; microscopy; field; study ... See more keywords
Visualization of ligand-induced dopamine D2S and D2L receptor internalization by TIRF microscopy
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-11436-1
Abstract: G protein-coupled receptors (GPCRs), including the dopamine receptors, represent a group of important pharmacological targets. Upon agonist binding, GPCRs frequently undergo internalization, a process that is known to attenuate functional responses upon prolonged exposure to… read more here.
Keywords: microscopy; internalization; tirf microscopy; receptor ... See more keywords