Hydrophobic and Hydrogel-Based Methods for Passive Tissue Clearing.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2051-9_12
Abstract: Optical tissue clearing enables the precise imaging of cellular and subcellular structures in whole organs and tissues without the need for physical tissue sectioning. By combining tissue clearing with confocal or lightsheet microscopy, 3D images… read more here.
Keywords: hydrophobic hydrogel; tissue; microscopy; hydrogel based ... See more keywords
Optical tissue clearing and immunolabeling in kidney research.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in cell biology"
DOI: 10.1016/bs.mcb.2019.07.001
Abstract: Optical clearing techniques have recently received wide attention for their potential to reveal microcellular structures deep within tissue. Traditional two-dimensional methods are limited for kidney research since renal tubules, glomeruli, vasculature, and other components exist… read more here.
Keywords: optical tissue; tissue clearing; kidney research; tissue ... See more keywords
Tissue clearing and imaging methods for cardiovascular development
Sign Up to like & getrecommendations! Published in 2021 at "iScience"
DOI: 10.1016/j.isci.2021.102387
Abstract: Summary Tissue imaging in 3D using visible light is limited and various clearing techniques were developed to increase imaging depth, but none provides universal solution for all tissues at all developmental stages. In this review,… read more here.
Keywords: clearing imaging; imaging methods; cardiovascular development; tissue ... See more keywords
Tissue Clearing To Localize Microplastics via Three-Dimensional Imaging of Whole Organisms.
Sign Up to like & getrecommendations! Published in 2023 at "Environmental science & technology"
DOI: 10.1021/acs.est.2c07209
Abstract: Understanding the biological impacts of plastic pollution requires an effective methodology to detect unlabeled microplastics in environmental samples. Detecting unlabeled microplastics in an organism generally requires a digestion protocol, which results in the loss of… read more here.
Keywords: tissue clearing; organism; unlabeled microplastics; microscopy ... See more keywords
Tissue Clearing and Its Application in the Musculoskeletal System
Sign Up to like & getrecommendations! Published in 2023 at "ACS Omega"
DOI: 10.1021/acsomega.2c05180
Abstract: The musculoskeletal system is an integral part of the human body. Currently, most skeletal muscle research is conducted through conventional histological sections due to technological limitations and the structure of skeletal muscles. For studying and… read more here.
Keywords: three dimensional; system; tissue clearing; musculoskeletal system ... See more keywords
Next generation histology methods for three-dimensional imaging of fresh and archival human brain tissues
Sign Up to like & getrecommendations! Published in 2018 at "Nature Communications"
DOI: 10.1038/s41467-018-03359-w
Abstract: Modern clearing techniques for the three-dimensional (3D) visualisation of neural tissue microstructure have been very effective when used on rodent brain but very few studies have utilised them on human brain material, mainly due to… read more here.
Keywords: three dimensional; human brain; tissue; tissue clearing ... See more keywords
descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques
Sign Up to like & getrecommendations! Published in 2024 at "Nature Communications"
DOI: 10.1038/s41467-024-49131-1
Abstract: Despite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktop-equipped SPIM for cleared specimens), a low-cost… read more here.
Keywords: sheet; tissue clearing; light sheet; sheet microscope ... See more keywords
SOLID: minimizing tissue distortion for brain-wide profiling of diverse architectures
Sign Up to like & getrecommendations! Published in 2024 at "Nature Communications"
DOI: 10.1038/s41467-024-52560-7
Abstract: Brain-wide profiling of diverse biological components is fundamental for understanding complex brain pathology. Despite the availability in whole-brain imaging, it is still challenging to conduct multiplexed, brain-wide analysis with current tissue clearing techniques. Here, we… read more here.
Keywords: brain; tissue clearing; tissue distortion; brain wide ... See more keywords
Three-dimensional Cardiomyocytes Structure Revealed By Diffusion Tensor Imaging and Its Validation Using a Tissue-Clearing Technique
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-24622-6
Abstract: We characterized the microstructural response of the myocardium to cardiovascular disease using diffusion tensor imaging (DTI) and performed histological validation by intact, un-sectioned, three-dimensional (3D) histology using a tissue-clearing technique. The approach was validated in… read more here.
Keywords: clearing technique; tissue clearing; histology;
Evaluation of tissue-clearing techniques for intraorgan imaging of distribution of polymeric nanoparticles as drug carriers
Sign Up to like & getrecommendations! Published in 2020 at "Drug Development and Industrial Pharmacy"
DOI: 10.1080/03639045.2020.1843476
Abstract: Abstract Objective The development of drug delivery systems using nanocarriers requires intraorgan imaging techniques for evaluating the distribution of nanocarriers. In this study, we evaluated the tissue-clearing techniques for the imaging of polymeric nanoparticles, a… read more here.
Keywords: polymeric nanoparticles; drug; modified cubic; intraorgan imaging ... See more keywords
Tissue clearing to examine glioma complexity in 3 dimensions.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of neuropathology and experimental neurology"
DOI: 10.1093/jnen/nlad024
Abstract: In recent years, tissue clearing has revolutionized the way we view biological materials. This has resulted in considerable advances in neuropathology and brain imaging. Its application to gliomas has the potential to increase understanding of… read more here.
Keywords: oncology; examine glioma; tissue clearing; complexity dimensions ... See more keywords