Getting the whole picture: High content screening using three‐dimensional cellular model systems and whole animal assays
Sign Up to like & getrecommendations! Published in 2017 at "Cytometry Part A"
DOI: 10.1002/cyto.a.22907
Abstract: Phenotypic or High Content Screening (HCS) is becoming more widely used for primary screening campaigns in drug discovery. Currently the vast majority of HCS campaigns are using cell lines grown in well‐established monolayer cultures (2D… read more here.
Keywords: animal assays; tissue culture; whole animal; content screening ... See more keywords
In vitro plant tissue culture: means for production of biological active compounds
Sign Up to like & getrecommendations! Published in 2018 at "Planta"
DOI: 10.1007/s00425-018-2910-1
Abstract: Main conclusionPlant tissue culture as an important tool for the continuous production of active compounds including secondary metabolites and engineered molecules. Novel methods (gene editing, abiotic stress) can improve the technique.Humans have a long history… read more here.
Keywords: tissue culture; active compounds; production; plant ... See more keywords
In vitro tissue culture model validation—the influence of tissue culture components on IPL energy output
Sign Up to like & getrecommendations! Published in 2019 at "Lasers in Medical Science"
DOI: 10.1007/s10103-019-02747-y
Abstract: Intense pulsed light (IPL) has been used therapeutically in a number of clinical settings and has been shown to have a photobiomodulatory effect on connective tissue cells, such as those derived from skin and tendon.… read more here.
Keywords: energy; components ipl; tissue culture; tissue ... See more keywords
Precise evaluation of tissue culture-induced variation during optimisation of in vitro regeneration regime in barley
Sign Up to like & getrecommendations! Published in 2020 at "Plant Molecular Biology"
DOI: 10.1007/s11103-020-00973-5
Abstract: Key message The Taguchi method and metAFLP analysis were used to optimise barley regenerants towards maximum and minimum levels of tissue culture-induced variation. The subtle effects of symmetric and asymmetric methylation changes in regenerants were… read more here.
Keywords: culture induced; variation; tissue culture; tissue ... See more keywords
Comparison between organismal staining on histology and tissue culture in the diagnosis of cutaneous infection: a retrospective study.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of the American Academy of Dermatology"
DOI: 10.1016/j.jaad.2020.01.047
Abstract: BACKGROUND In instances of suspected cutaneous infection, standard of care includes obtaining skin biopsies for histology and tissue culture. Few studies have compared the clinical utility of each test. OBJECTIVE To assess the concordance of… read more here.
Keywords: histology tissue; tissue culture; infection; histology ... See more keywords
Current progress and challenges in crop genetic transformation.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of plant physiology"
DOI: 10.1016/j.jplph.2021.153411
Abstract: Plant transformation remains the most sought-after technology for functional genomics and crop genetic improvement, especially for introducing specific new traits and to modify or recombine already existing traits. Along with many other agricultural technologies, the… read more here.
Keywords: plant; crop genetic; current progress; tissue culture ... See more keywords
A Comparative study of direct fluorescent antibody, mouse inoculation, and tissue culture infection testing for Rabies diagnoses.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of virological methods"
DOI: 10.1016/j.jviromet.2021.114426
Abstract: The laboratory diagnosis of rabies is of fundamental importance to the evaluation of suspected cases of rabies virus (RABV) infection. Confirmation of direct fluorescent antibody test (DFAT) results via viral isolation (VI) is recommended, and… read more here.
Keywords: fluorescent antibody; culture infection; infection; mouse inoculation ... See more keywords
Resveratrol supports and alpha‐naphthoflavone disrupts growth of human ovarian follicles in an in vitro tissue culture model
Sign Up to like & getrecommendations! Published in 2018 at "Toxicology and Applied Pharmacology"
DOI: 10.1016/j.taap.2017.11.009
Abstract: ABSTRACT Infertility is a global health problem with an estimated incidence of 15%. Exposure to chemicals is a potential causal factor, and there is a lack of studies examining the effects on female germ cells.… read more here.
Keywords: tissue culture; tissue; ovarian follicles; human ovarian ... See more keywords
Spontaneous Formation of 3D Breast Cancer Tissues on Electrospun Chitosan/Poly(ethylene oxide) Nanofibrous Scaffolds
Sign Up to like & getrecommendations! Published in 2022 at "ACS Omega"
DOI: 10.1021/acsomega.1c05646
Abstract: Three-dimensional (3D) tissue culture has attracted a great deal of attention as a result of the need to replace the conventional two-dimensional cell cultures with more meaningful methods, especially for understanding the sophisticated nature of… read more here.
Keywords: culture; tissue culture; breast cancer; nanofibrous scaffolds ... See more keywords
Evaluation of Silk Inverse Opals for “Smart” Tissue Culture
Sign Up to like & getrecommendations! Published in 2017 at "ACS Omega"
DOI: 10.1021/acsomega.6b00320
Abstract: Visually tracking the subtle aspects of biological systems in real time during tissue culture remains challenging. Herein, we demonstrate the use of bioactive, cytocompatible, and biodegradable inverse opals from silk as a multifunctional substrate to… read more here.
Keywords: tissue culture; evaluation silk; tissue; inverse opals ... See more keywords
Inducible Transposition of a Heat-Activated Retrotransposon in Tissue Culture
Sign Up to like & getrecommendations! Published in 2017 at "Plant and Cell Physiology"
DOI: 10.1093/pcp/pcw202
Abstract: A transposition of a heat-activated retrotransposon named ONSEN required compromise of a small RNA-mediated epigenetic regulation that includes RNA-directed DNA methylation (RdDM) machinery after heat treatment. In the current study, we analyzed the transcriptional and… read more here.
Keywords: heat; tissue culture; transposition heat;