High‐throughput proteomic analysis of FFPE tissue samples facilitates tumor stratification
Sign Up to like & getrecommendations! Published in 2019 at "Molecular Oncology"
DOI: 10.1002/1878-0261.12570
Abstract: Formalin‐fixed, paraffin‐embedded (FFPE), biobanked tissue samples offer an invaluable resource for clinical and biomarker research. Here, we developed a pressure cycling technology (PCT)‐SWATH mass spectrometry workflow to analyze FFPE tissue proteomes and applied it to… read more here.
Keywords: stratification; ffpe samples; tissue samples; ffpe ... See more keywords
UltraPlex Hapten-Based Multiplexed Fluorescent Immunohistochemistry.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1593-5_17
Abstract: The UltraPlex method for multiplexed two-dimensional fluorescent immunohistochemistry is described, in which hapten tags conjugated to primary antibodies facilitate multiplexed imaging of four or more antigens per tissue section at once. Anti-hapten secondary antibodies labeled… read more here.
Keywords: ultraplex hapten; immunohistochemistry; tissue samples; hapten based ... See more keywords
Preparation of Tissue Samples for Large-scale Quantitative Mass Spectrometric Analysis
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology and Bioprocess Engineering"
DOI: 10.1007/s12257-019-0495-6
Abstract: Tissues contain more tumor-type specific information than biofluids, such as blood, rendering them valuable resources for biomarker studies. However, considering the characteristics of tissue homogenization, it is difficult to obtain reproducible samples and analyze many… read more here.
Keywords: large scale; preparation tissue; tissue samples; analysis ... See more keywords
Measurement of lysyl oxidase activity from small tissue samples and cell cultures.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in cell biology"
DOI: 10.1016/bs.mcb.2017.08.009
Abstract: Increasing interest in the multifunctional lysyl oxidase family of proteins is evident from the growth in the number of new publications each year. The enzymes have unique properties of strong affinities to extracellular matrix components,… read more here.
Keywords: tissue samples; samples cell; oxidase activity; lysyl oxidase ... See more keywords
Development of duplex TaqMan-based real-time PCR assay for the simultaneous detection of Perkinsus olseni and P. chesapeaki in host Manila clam tissue samples.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of invertebrate pathology"
DOI: 10.1016/j.jip.2021.107603
Abstract: The aetiological agent Perkinsus olseni is globally recognised as a major threat for shellfish production considering its wide geographical distribution across Asia, Europe, Australia and South America. Another species, Perkinsus chesapeaki, which has never been… read more here.
Keywords: tissue samples; duplex; olseni chesapeaki; perkinsus olseni ... See more keywords
Estimation of the Young's moduli of fresh human oropharyngeal soft tissues using indentation testing.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of the mechanical behavior of biomedical materials"
DOI: 10.1016/j.jmbbm.2018.07.004
Abstract: Finite element (FE)-based biomechanical simulations of the upper airway are promising computational tools to study abnormal upper airway deformations under obstructive sleep apnea (OSA) conditions and to help guide minimally invasive surgical interventions in case… read more here.
Keywords: young moduli; tissue samples; oropharyngeal soft; soft tissues ... See more keywords
“Two is not enough” – Impact of the number of tissue samples obtained from stereotactic brain biopsies in suspected glioblastoma
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Clinical Neuroscience"
DOI: 10.1016/j.jocn.2017.09.032
Abstract: OBJECTIVE Stereotactic procedures are performed in many neurosurgical departments in order to obtain tumor tissue from brain lesions for histopathological evaluation. Biopsies can be performed frame-guided and frame less. Some departments use a biopsy needle… read more here.
Keywords: tissue samples; diagnosis; suspected glioblastoma; group ... See more keywords
Culturing Explants for Cutibacterium at Revision Shoulder Arthroplasty: An Analysis of Explant and Tissue Samples at Corresponding Anatomic Sites.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of shoulder and elbow surgery"
DOI: 10.1016/j.jse.2022.03.004
Abstract: BACKGROUND Culturing of deep tissues obtained at revision arthroplasty for Cutibacterium is a key component of diagnosing a periprosthetic infection. The value of culturing explanted components has not been well-described. This study sought to answer… read more here.
Keywords: cutibacterium; shoulder; revision; tissue ... See more keywords
Comparison of different digestion methods for proteomic analysis of isolated cells and FFPE tissue samples.
Sign Up to like & getrecommendations! Published in 2021 at "Talanta"
DOI: 10.1016/j.talanta.2021.122568
Abstract: Proteomics of human tissues and isolated cellular subpopulations create new opportunities for therapy and monitoring of a patients' treatment in the clinic. Important considerations in such analysis include recovery of adequate amounts of protein for… read more here.
Keywords: tissue samples; analysis; digestion; method ... See more keywords
Circular DNA viruses identified in short-finned pilot whale and orca tissue samples.
Sign Up to like & getrecommendations! Published in 2021 at "Virology"
DOI: 10.1016/j.virol.2021.04.004
Abstract: Members of the Delphinidae family are widely distributed across the world's oceans. We used a viral metagenomic approach to identify viruses in orca (Orcinus orca) and short-finned pilot whale (Globicephala macrorhynchus) muscle, kidney, and liver… read more here.
Keywords: short finned; orca tissue; tissue samples; finned pilot ... See more keywords
Optimization of coated blade spray for rapid screening and quantitation of 105 veterinary drugs in biological tissue samples.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c00093
Abstract: Rapid and efficient determination of contaminants at trace levels in tissue samples has become an unmet need around the globe. Coated Blade Spray (CBS) extraction/ionization is a technology capable of performing, with a single device,… read more here.
Keywords: blade spray; tissue samples; cbs; tissue ... See more keywords