Identifying prognostic structural features in tissue sections of colon cancer patients using point pattern analysis.
Sign Up to like & getrecommendations! Published in 2019 at "Statistics in medicine"
DOI: 10.1002/sim.8046
Abstract: Diagnosis and prognosis of cancer are informed by the architecture inherent in cancer patient tissue sections. This architecture is typically identified by pathologists, yet advances in computational image analysis facilitate quantitative assessment of this structure.… read more here.
Keywords: analysis; point; tissue sections; prognostic structural ... See more keywords
On-tissue amidation of sialic acid with aniline for sensitive imaging of sialylated N-glycans from FFPE tissue sections via MALDI mass spectrometry
Sign Up to like & getrecommendations! Published in 2022 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-022-03894-y
Abstract: Spatial visualization of glycans within clinical tissue samples is critical for discovery of disease-relevant glycan dysregulations. Herein, we develop an on-tissue derivatization strategy for sensitive spatial visualization of N-glycans from formalin-fixed paraffin-embedded (FFPE) tissue sections,… read more here.
Keywords: tissue sections; tissue; sialic acid; amidation ... See more keywords
Joint reconstruction and classification of tumor cells and cell interactions in melanoma tissue sections with synthesized training data
Sign Up to like & getrecommendations! Published in 2019 at "International Journal of Computer Assisted Radiology and Surgery"
DOI: 10.1007/s11548-019-01919-z
Abstract: PurposeCancers are almost always diagnosed by morphologic features in tissue sections. In this context, machine learning tools provide new opportunities to describe tumor immune cell interactions within the tumor microenvironment and thus provide phenotypic information… read more here.
Keywords: classification; training data; tissue; cell ... See more keywords
Methods for histological characterization of cryo-induced myocardial infarction in a rat model.
Sign Up to like & getrecommendations! Published in 2020 at "Acta histochemica"
DOI: 10.1016/j.acthis.2020.151624
Abstract: Ligation of the left anterior descending (LAD) coronary artery has been commonly employed to induce myocardial infarction (MI) in animals; however, it is known to pose setbacks in the form of cardiac arrhythmias and unpredictable… read more here.
Keywords: cryo induced; myocardial infarction; infarction; model ... See more keywords
Storage conditions of human kidney tissue sections affect spatial lipidomics analysis reproducibility.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1021/jasms.0c00256
Abstract: Lipids often are labile, unstable, and tend to degrade overtime, so it is of upmost importance to study these molecules in their most native state. We sought to understand the optimal storage conditions for spatial… read more here.
Keywords: kidney tissue; storage conditions; tissue sections; tissue ... See more keywords
Infrared Laser Ablation Microsampling with a Reflective Objective
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1021/jasms.1c00306
Abstract: A Schwarzschild reflective objective with a numerical aperture of 0.3 and working distance of 10 cm was used for laser ablation sampling of tissue for off-line mass spectrometry. The objective focused the laser to a… read more here.
Keywords: mass spectrometry; tissue sections; laser ablation; reflective objective ... See more keywords
On-Tissue Chemical Derivatization for Comprehensive Mapping of Brain Carboxyl and Aldehyde Metabolites by MALDI–MS Imaging
Sign Up to like & getrecommendations! Published in 2023 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1021/jasms.2c00336
Abstract: The visualization of small metabolites by MALDI mass spectrometry imaging in brain tissue sections is challenging due to low detection sensitivity and high background interference. We present an on-tissue chemical derivatization MALDI mass spectrometry imaging… read more here.
Keywords: brain; brain tissue; tissue sections; derivatization ... See more keywords
Rapid brain structure and tumour margin detection on whole frozen tissue sections by fast multiphotometric mid-infrared scanning
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-90777-4
Abstract: Frozen section analysis is a frequently used method for examination of tissue samples, especially for tumour detection. In the majority of cases, the aim is to identify characteristic tissue morphologies or tumour margins. Depending on… read more here.
Keywords: detection; whole frozen; tissue; mid infrared ... See more keywords
A novel excision selection method for isolation of antibodies binding antigens expressed specifically by rare cells in tissue sections
Sign Up to like & getrecommendations! Published in 2017 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkx207
Abstract: Abstract There is a growing appreciation of single cell technologies to provide increased biological insight and allow development of improved therapeutics. The central dogma explains why single cell technologies is further advanced in studies targeting… read more here.
Keywords: cells tissue; selection; antigens expressed; rare cells ... See more keywords
Automated and Reproducible Detection of Vascular Endothelial Growth Factor (VEGF) in Renal Tissue Sections
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Immunology Research"
DOI: 10.1155/2019/7232781
Abstract: Background Manual analysis of tissue sections, such as for pathological diagnosis, requires an analyst with substantial knowledge and experience. Reproducible image analysis of biological samples is steadily gaining scientific importance. The aim of the present… read more here.
Keywords: vascular endothelial; endothelial growth; factor vegf; tissue ... See more keywords
Abstract 3434: Digital spatial profiling platform allows for spatially resolved, high-plex quantification of mRNA distribution and abundance on FFPE and fresh frozen tissue sections
Sign Up to like & getrecommendations! Published in 2018 at "Cancer Research"
DOI: 10.1158/1538-7445.am2018-3434
Abstract: Characterization of spatial mRNA expression across multiple targets in a single tissue section has proven difficult to accomplish. To address this unmet need, we have developed a novel imaging platform, Digital Spatial Profiling (DSP), designed… read more here.
Keywords: ffpe fresh; high plex; fresh frozen; tissue ... See more keywords