Isothermal Titration Calorimetry Assays to Measure Binding Affinities In Vitro.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8910-2_19
Abstract: In the study of the Hippo signal transduction pathway, protein-protein interactions are often explored, because various proteins such as MOB1, NDR1/NDR2, and LATS1/LATS2 are very important members in this complicated signaling pathway. The transduction of… read more here.
Keywords: protein; isothermal titration; titration calorimetry; protein protein ... See more keywords
Repeatability, precision, and accuracy of the enthalpies and Gibbs energies of a protein–ligand binding reaction measured by isothermal titration calorimetry
Sign Up to like & getrecommendations! Published in 2018 at "European Biophysics Journal"
DOI: 10.1007/s00249-018-1341-z
Abstract: In rational drug design, it is important to determine accurately and with high precision the binding constant (the affinity or the change in Gibbs energy, ∆G), the change in enthalpy (ΔH), and the entropy change… read more here.
Keywords: accuracy; isothermal titration; titration calorimetry; protein ligand ... See more keywords
Spectroscopic and Isothermal Titration Calorimetry Studies of Binding Interactions Between Carbon Nanodots and Serum Albumins
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Solution Chemistry"
DOI: 10.1007/s10953-018-0792-2
Abstract: Carbon nanodots (C-dots) have attracted great attention as a new class of luminescent nanomaterials. In order to better understand the basic behavior of C-dots in biological systems, the binding characteristics of C-dots with bovine serum… read more here.
Keywords: spectroscopic isothermal; carbon nanodots; titration calorimetry; isothermal titration ... See more keywords
Differential scanning fluorimetry followed by microscale thermophoresis and/or isothermal titration calorimetry as an efficient tool for ligand screening
Sign Up to like & getrecommendations! Published in 2025 at "Biophysical Reviews"
DOI: 10.1007/s12551-025-01280-3
Abstract: Various biophysical and biochemical techniques have been developed to measure the affinity of interacting molecules. This review analyzes the combination of three methods: differential scanning fluorimetry as the initial high-throughput screening technique and microscale thermophoresis… read more here.
Keywords: thermophoresis isothermal; isothermal titration; titration calorimetry; microscale thermophoresis ... See more keywords
Real-time monitoring of D-Ala-D-Ala dipeptidase activity of VanX in living bacteria by isothermal titration calorimetry.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2019.05.002
Abstract: The d,d-dipeptidase enzyme VanX is the main cause of vancomycin resistance in gram-positive bacteria because of hydrolysis of the D-Ala-D-Ala dipeptide used in cell-wall biosynthesis. Continuous assay of VanX has proven challenging due to lack… read more here.
Keywords: ala ala; dipeptidase; titration calorimetry; isothermal titration ... See more keywords
Corrigendum to "Isothermal Titration Calorimetry and Surface Plasmon Resonance Analysis Using the Dynamic Approach".
Sign Up to like & getrecommendations! Published in 2020 at "Biochemistry and biophysics reports"
DOI: 10.1016/j.bbrep.2020.100800
Abstract: [This corrects the article DOI: 10.1016/j.bbrep.2019.100712.]. read more here.
Keywords: calorimetry surface; surface plasmon; isothermal titration; plasmon resonance ... See more keywords
Nucleotide coordination with 14 lanthanides studied by isothermal titration calorimetry
Sign Up to like & getrecommendations! Published in 2018 at "Chinese Chemical Letters"
DOI: 10.1016/j.cclet.2017.06.014
Abstract: Abstract With their hydrolytic, optical and magnetic properties, lanthanide ions (Ln 3+ ) are versatile probes for nucleic acids. In addition, nucleotide-coordinated Ln 3+ ions form useful nanoparticles. However, the thermodynamic basis of their interaction… read more here.
Keywords: nucleotide coordination; coordination lanthanides; titration calorimetry; isothermal titration ... See more keywords
Molecular design and engineering for affinity separation processes using isothermal titration calorimetry (ITC) and molecular modeling (MM)
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Molecular Liquids"
DOI: 10.1016/j.molliq.2019.03.060
Abstract: Abstract A molecular design approach based on isothermal titration calorimetry (ITC) and molecular modeling (MM) was investigated for the development of new extractants in liquid-liquid extraction (LLX) systems. The molecular designs were validated in LLX… read more here.
Keywords: isothermal titration; molecular design; titration calorimetry; itc molecular ... See more keywords
Measuring Rapid Time-Scale Reaction Kinetics Using Isothermal Titration Calorimetry.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.7b00693
Abstract: Isothermal titration calorimetry (ITC) is a powerful tool for acquiring both thermodynamic and kinetic data for biological interactions including molecular recognition and enzymatic catalysis. ITC-based kinetics measurements typically focus on reactions taking place over long… read more here.
Keywords: itc; time; titration calorimetry; isothermal titration ... See more keywords
Fast and robust quantification of detergent micellization thermodynamics from isothermal titration calorimetry.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.9b04281
Abstract: Detergents are widely used in modern in vitro biochemistry and biophysics, in particular to aid the characterization of integral membrane proteins. An important characteristic of these chemicals in aqueous solutions is the concentration above which… read more here.
Keywords: thermodynamics; titration calorimetry; isothermal titration; micellization ... See more keywords
Isothermal Titration Calorimetry Study of Brine–Oil–Rock Interactions
Sign Up to like & getrecommendations! Published in 2018 at "Energy & Fuels"
DOI: 10.1021/acs.energyfuels.8b00512
Abstract: Isothermal titration calorimetry (ITC) is a technique that allows us to accurately determine the thermodynamic parameters that characterize a binding interaction between two molecular systems. However, this technique has not had a wide application in… read more here.
Keywords: brine oil; titration calorimetry; oil; isothermal titration ... See more keywords