Optimal analytical strategies for sensitive and quantitative phosphoproteomics using TMT‐based multiplexing
Sign Up to like & getrecommendations! Published in 2022 at "PROTEOMICS"
DOI: 10.1002/pmic.202100245
Abstract: In large‐scale quantitative mass spectrometry (MS)‐based phosphoproteomics, isobaric labeling with tandem mass tags (TMTs) coupled with offline high‐pH reversed‐phase peptide chromatographic fractionation maximizes depth of coverage. To investigate to what extent limited sample amounts affect… read more here.
Keywords: tmt based; sample; peptide input; per sample ... See more keywords
Physiological and TMT-based proteomic analysis of oat early seedlings in response to alkali stress.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of proteomics"
DOI: 10.1016/j.jprot.2018.12.018
Abstract: Oats are an important cereal crop worldwide, and they also serve as a phytoremediation crop to ameliorate salinized and alkalized soils. However, the mechanism of the oat response to alkali remains unclear. Physiological and tandem… read more here.
Keywords: response alkali; tmt based; stress; alkali stress ... See more keywords
TMT-Based Multiplexed Quantitation of N-Glycopeptides Reveals Glycoproteome Remodeling Induced by Oncogenic Mutations
Sign Up to like & getrecommendations! Published in 2022 at "ACS Omega"
DOI: 10.1021/acsomega.1c06970
Abstract: Glycoproteomics, or the simultaneous characterization of glycans and their attached peptides, is increasingly being employed to generate catalogs of glycopeptides on a large scale. Nevertheless, quantitative glycoproteomics remains challenging even though isobaric tagging reagents such… read more here.
Keywords: based multiplexed; tmt based; multiplexed quantitation; oncogenic mutations ... See more keywords
TMT-based proteomic analysis of endoplasmic reticulum proteins in mulberry leaves under UV-B and dark stress.
Sign Up to like & getrecommendations! Published in 2022 at "Physiologia plantarum"
DOI: 10.1111/ppl.13667
Abstract: Mulberry leaves have been used in traditional Chinese medicine due to their antioxidant, antidiabetic, and antihyperlipidemic properties. A previous study showed that ultraviolet-B radiation followed by dark incubation could improve the contents of active ingredients… read more here.
Keywords: mulberry leaves; stress; tmt based; protein ... See more keywords
TMT-based quantitative proteomics analysis reveals the role of Notch signaling in FAdV-4-infected LMH cell
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.988259
Abstract: Fowl adenovirus serotype 4 (FAdV-4) is recognized as a pathogen that causes hydropericardium syndrome. Irrespective of the pathway used by the virus to invade the chicken, the pathological characteristics of the disease include degeneration and… read more here.
Keywords: tmt based; notch signaling; lmh; analysis ... See more keywords
TMT-Based Quantitative Proteomic Profiling of Overwintering Lissorhoptrus oryzophilus
Sign Up to like & getrecommendations! Published in 2019 at "Frontiers in Physiology"
DOI: 10.3389/fphys.2019.01623
Abstract: Adaptations to low temperature play a critical role in restricting the geographical distribution of insects. Decreasing day lengths and temperatures trigger seasonal cold adaptations in insects. These adaptions include changes in expression at the miRNA,… read more here.
Keywords: quantitative proteomic; insects; tmt based; based quantitative ... See more keywords
Proteomic Exploration of Porcine Oocytes During Meiotic Maturation in vitro Using an Accurate TMT-Based Quantitative Approach
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Veterinary Science"
DOI: 10.3389/fvets.2021.792869
Abstract: The dynamic changes in protein expression are well known to be required for oocyte meiotic maturation. Although proteomic analysis has been performed in porcine oocytes during in vitro maturation, there is still no full data… read more here.
Keywords: quantitative approach; tmt based; maturation; meiotic maturation ... See more keywords
One Step Forward with Dry Surface Biofilm (DSB) of Staphylococcus aureus: TMT-Based Quantitative Proteomic Analysis Reveals Proteomic Shifts between DSB and Hydrated Biofilm
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms232012238
Abstract: The Gram-positive bacterium Staphylococcus aureus is responsible for serious acute and chronic infections worldwide and is well-known for its biofilm formation ability. Recent findings of biofilms on dry hospital surfaces emphasise the failures in current… read more here.
Keywords: tmt based; biofilm; analysis; hydrated biofilm ... See more keywords
TMT-Based Proteomic Analysis of Continuous Cropping Response in Codonopsis tangshen Oliv.
Sign Up to like & getrecommendations! Published in 2023 at "Life"
DOI: 10.3390/life13030765
Abstract: The growth and development of Codonopsis tangshen, an important herb used in Chinese traditional medicine, have been seriously affected by continuous cropping obstacles. Therefore, understanding the molecular responses of C. tangshen to continuous cropping is… read more here.
Keywords: tmt based; cropping obstacles; codonopsis tangshen; tangshen ... See more keywords
Serum Proteomic Analysis by Tandem Mass Tags (TMT) Based Quantitative Proteomics in Gastric Cancer Patients.
Sign Up to like & getrecommendations! Published in 2018 at "Clinical laboratory"
DOI: 10.7754/clin.lab.2018.171129
Abstract: BACKGROUND To use the tandem mass tags (TMT) based quantitative proteomics technique and bioinformatics method to identify potential serum diagnostic biomarkers for gastric cancer (GC). METHODS This study enrolled GC patients and healthy control subjects.… read more here.
Keywords: mass tags; tmt based; based quantitative; quantitative proteomics ... See more keywords