Dual-Mode SERS and Electrochemical Detection of miRNA Based on Popcorn-like Gold Nanofilms and Toehold-Mediated Strand Displacement Amplification Reaction.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c05221
Abstract: MicroRNA (miRNA) has emerged as one of the ideal target biomarker analytes for cancer detection because its abnormal expression is closely related to the occurrence of many cancers. In this work, we combined three-dimensional (3D)… read more here.
Keywords: popcorn like; detection; toehold mediated; like gold ... See more keywords
Logic Circuits for Intelligent Microcystin Monitoring Based on Aptamer Recognition and Toehold-Mediated Hairpin DNA Self-Assembly.
Sign Up to like & getrecommendations! Published in 2025 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.4c06655
Abstract: A sensitive fluorescence biosensor was developed for microcystin-LR (MC-LR) detection using H1, H2, and H3 DNA probes as sensing elements. The aptamer in H1 can recognize the target. H2 was labeled with FAM and BHQ.… read more here.
Keywords: circuit; toehold mediated; logic; self assembly ... See more keywords
A PAM-free CRISPR/Cas12a ultra-specific activation mode based on toehold-mediated strand displacement and branch migration
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac886
Abstract: Abstract CRISPR (clustered regularly interspaced short palindromic repeats) technology has achieved great breakthroughs in terms of convenience and sensitivity; it is becoming the most promising molecular tool. However, only two CRISPR activation modes (single and… read more here.
Keywords: crispr cas12a; crispr; toehold mediated; activation mode ... See more keywords
Photocontrolled dissociation and toehold-mediated strand displacement-based synergistic regulation of CRISPR-Cas12a
Sign Up to like & getrecommendations! Published in 2025 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkaf1178
Abstract: Abstract The trans-cleavage activity of the CRISPR-Cas system holds broad potential across diverse fields, yet precise spatiotemporal regulation remains challenging due to the predominantly single-direction control strategies available. Here, we present a bidirectional, multi-round modulation… read more here.
Keywords: toehold mediated; strand displacement; crispr cas12a; mediated strand ... See more keywords