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   Articles with "top proteomics" as a keyword



Large‐scale top‐down proteomics of the Arabidopsis thaliana leaf and chloroplast proteomes

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recommendations! Published in 2022 at "PROTEOMICS"

DOI: 10.1002/pmic.202100377

Abstract: We present a large‐scale top‐down proteomics (TDP) study of plant leaf and chloroplast proteins, achieving the identification of over 4700 unique proteoforms. Using capillary zone electrophoresis coupled with tandem mass spectrometry analysis of offline size‐exclusion… read more here.

Keywords: large scale; top proteomics; scale top; leaf chloroplast ... See more keywords

Spectral averaging with outlier rejection algorithms to increase identifications in top-down proteomics

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recommendations! Published in 2024 at "Proteomics"

DOI: 10.1002/pmic.202300234

Abstract: The identification of proteoforms by top‐down proteomics requires both high quality fragmentation spectra and the neutral mass of the proteoform from which the fragments derive. Intact proteoform spectra can be highly complex and may include… read more here.

Keywords: top proteomics; rejection algorithms; rejection; outlier rejection ... See more keywords
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The benefits (and misfortunes) of SDS in top-down proteomics.

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recommendations! Published in 2018 at "Journal of proteomics"

DOI: 10.1016/j.jprot.2017.03.002

Abstract: Top-down proteomics (TDP) has great potential for high throughput proteoform characterization. With significant advances in mass spectrometry (MS) instrumentation permitting tandem MS of large intact proteins, a limitation to the widespread adoption of TDP still… read more here.

Keywords: misfortunes sds; intact proteins; proteomics benefits; top proteomics ... See more keywords
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Sensitive Top-Down Proteomics Analysis of a Low Number of Mammalian Cells Using a Nanodroplet Sample Processing Platform.

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recommendations! Published in 2020 at "Analytical chemistry"

DOI: 10.1021/acs.analchem.0c00467

Abstract: Top-down proteomics is a powerful tool for characterizing genetic variations and post-translational modifications at intact protein level. However, one significant technical gap of top-down proteomics is the inability to analyze a low amount of biological… read more here.

Keywords: sample; proteomics analysis; sensitive top; platform ... See more keywords

Size-Based Proteome Fractionation through Polyacrylamide Gel Electrophoresis Combined with LC-FAIMS-MS for In-Depth Top-Down Proteomics.

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recommendations! Published in 2022 at "Analytical chemistry"

DOI: 10.1021/acs.analchem.2c02777

Abstract: The combination of liquid chromatography (LC) and gas-phase separation by field-asymmetric ion mobility spectrometry (FAIMS) is a powerful proteoform separation system for top-down proteomics. Here, we present an in-depth top-down proteomics workflow, GeLC-FAIMS-MS, in which… read more here.

Keywords: top proteomics; polyacrylamide gel; fractionation; based proteome ... See more keywords

High-Throughput Top-Down Proteomic Analysis of Picogram-Level Complex Samples Using Multisegment Spray-Capillary CE-MS.

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recommendations! Published in 2025 at "Analytical chemistry"

DOI: 10.1021/acs.analchem.5c02334

Abstract: Ultrasensitive top-down proteomics techniques provide valuable insights into PTM-regulated cellular functions in mass-limited samples. Capillary electrophoresis (CE) is a promising separation technique for top-down proteomics due to its high resolution, high sensitivity, and short cycle… read more here.

Keywords: spray capillary; top proteomics; analysis; mass limited ... See more keywords
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Capillary HILIC-MS: A New Tool for Sensitive Top-Down Proteomics

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recommendations! Published in 2018 at "Analytical Chemistry"

DOI: 10.1021/acs.analchem.8b00382

Abstract: Recent progress in top-down proteomics has driven the demand for chromatographic methods compatible with mass spectrometry (MS) that can separate intact proteins. Hydrophilic interaction liquid chromatography (HILIC) has recently shown good potential for the characterization… read more here.

Keywords: new tool; capillary hilic; tool sensitive; analysis ... See more keywords

MASH Explorer: A Universal Software Environment for Top-Down Proteomics.

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recommendations! Published in 2020 at "Journal of proteome research"

DOI: 10.1021/acs.jproteome.0c00469

Abstract: Top-down mass spectrometry (MS)-based proteomics enables a comprehensive analysis of proteoforms with molecular specificity to achieve a proteome-wide understanding of protein functions. However, the lack of a universal software for top-down proteomics is becoming increasingly… read more here.

Keywords: top proteomics; universal software; explorer universal; mash explorer ... See more keywords

Validation of Top-Down Proteomics Data by Bottom-Up-Based N-Terminomics Reveals Pitfalls in Top-Down-Based Terminomics Workflows.

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recommendations! Published in 2022 at "Journal of proteome research"

DOI: 10.1021/acs.jproteome.2c00277

Abstract: Bottom-up proteomics (BUP)-based N-terminomics techniques have become standard to identify protein N-termini. While these methods rely on the identification of N-terminal peptides only, top-down proteomics (TDP) comes with the promise to provide additional information about… read more here.

Keywords: top proteomics; proteomics data; data bottom; based terminomics ... See more keywords

TopPICR: A Companion R Package for Top-Down Proteomics Data Analysis.

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recommendations! Published in 2023 at "Journal of proteome research"

DOI: 10.1021/acs.jproteome.2c00570

Abstract: Top-down proteomics is the analysis of proteins in their intact form without proteolysis, thus preserving valuable information about post-translational modifications, isoforms, and proteolytic processing. However, it is still a developing field due to limitations in… read more here.

Keywords: top proteomics; toppicr; companion package; data analysis ... See more keywords

Defining the Sarcomeric Proteoform Landscape in Ischemic Cardiomyopathy by Top-Down Proteomics.

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recommendations! Published in 2023 at "Journal of proteome research"

DOI: 10.1021/acs.jproteome.2c00729

Abstract: Ischemic cardiomyopathy (ICM) is a prominent form of heart failure, but the molecular mechanisms underlying ICM remain relatively understudied due to marked phenotypic heterogeneity. Alterations in post-translational modifications (PTMs) and isoform switches in sarcomeric proteins… read more here.

Keywords: icm; top proteomics; sarcomeric proteoform; proteoform landscape ... See more keywords