Characterization of the cervical bacterial community in dairy cows with metritis and during different physiological phases.
Sign Up to like & getrecommendations! Published in 2018 at "Theriogenology"
DOI: 10.1016/j.theriogenology.2017.12.028
Abstract: For the development of disease prevention and intervention strategies, a better understanding of the dynamics and interactions within cervical bacterial communities in both healthy cows and cows with metritis is required. Understanding the complexity and… read more here.
Keywords: community; cervical bacterial; physiological phases; total microbial ... See more keywords
The guanidine thiocyanate‐high EDTA method for total microbial RNA extraction from severely heavy metal‐contaminated soils
Sign Up to like & getrecommendations! Published in 2020 at "Microbial Biotechnology"
DOI: 10.1111/1751-7915.13615
Abstract: Molecular analyses relying on RNA, as a direct way to unravel active microbes and their functional genes, have received increasing attention from environmental researchers recently. However, extracting sufficient and high‐quality total microbial RNA from seriously… read more here.
Keywords: method; contaminated soils; total microbial; microbial rna ... See more keywords
Equivolumetric Protocol Generates Library Sizes Proportional to Total Microbial Load in 16S Amplicon Sequencing
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2021.638231
Abstract: High-throughput sequencing of 16S rRNA amplicon has been extensively employed to perform microbiome characterization worldwide. As a culture-independent methodology, it has allowed high-level profiling of sample bacterial composition directly from samples. However, most studies are… read more here.
Keywords: microbial load; equivolumetric protocol; microbiology; total microbial ... See more keywords