Isolation of Mitochondria from Liver and Extraction of Total RNA and Protein: Analyses of miRNA and Protein Expression.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1433-4_1
Abstract: Several studies have indicated the presence of microRNAs (miRNAs) within mitochondria although the origin, as well as the biological function, of these mitochondrially located miRNAs is largely unknown. The identification and significance of this subcellular… read more here.
Keywords: protein; rna; total rna; extraction total ... See more keywords
Improved method for isolation of high-quality total RNA from Agave tequilana Weber roots
Sign Up to like & getrecommendations! Published in 2021 at "3 Biotech"
DOI: 10.1007/s13205-020-02620-8
Abstract: Together with their undeniable role in the ecology of arid and semiarid ecosystems, Agave species are emerging as a model to dissect the relationships between crassulacean acid metabolism and high efficiency of light and water… read more here.
Keywords: quality; agave tequilana; high quality; quality total ... See more keywords
Graphene Oxide as a Bifunctional Material toward Superior RNA Protection and Extraction.
Sign Up to like & getrecommendations! Published in 2018 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.8b12522
Abstract: It is well known that graphene oxide (GO), a planar nanomaterial, is endowed with the capacity to immobilize short ssRNA via π-π stacking, thus enhancing its stability. However, whether large RNA molecules, such as total… read more here.
Keywords: graphene oxide; protection; oxide bifunctional; rna ... See more keywords
Performance assessment of total RNA sequencing of human biofluids and extracellular vesicles
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-53892-x
Abstract: RNA profiling has emerged as a powerful tool to investigate the biomarker potential of human biofluids. However, despite enormous interest in extracellular nucleic acids, RNA sequencing methods to quantify the total RNA content outside cells… read more here.
Keywords: extracellular vesicles; human biofluids; rna; rna sequencing ... See more keywords
Detection of RNA viruses in Cape gooseberry (Physalis peruviana L.) by RNAseq using total RNA and dsRNA inputs
Sign Up to like & getrecommendations! Published in 2020 at "Archives of Phytopathology and Plant Protection"
DOI: 10.1080/03235408.2020.1748368
Abstract: Abstract Physalis peruviana L. is a herbaceous solanaceous plant native to the South American Andes that produces a small yellow fruit of about 1-2 cm in diameter with high demand in the international markets. Currently, Colombia… read more here.
Keywords: potato; rna; physalis peruviana; dsrna ... See more keywords
189 Comparative transcriptomic analysis between Total RNA and mRNA isolated from feces of neonatal dairy calves
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Animal Science"
DOI: 10.1093/jas/skz122.195
Abstract: The fecal RNA method can be used to evaluate biological adaptations of the gastrointestinal tract of dairy calves through gene expression analysis. The process of RNA isolation from fecal samples presents several challenges, including the… read more here.
Keywords: rna; total rna; rna mrna; dairy calves ... See more keywords
Metagenomics versus total RNA sequencing: most accurate data-processing tools, microbial identification accuracy and perspectives for ecological assessments
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac689
Abstract: Abstract Metagenomics and total RNA sequencing (total RNA-Seq) have the potential to improve the taxonomic identification of diverse microbial communities, which could allow for the incorporation of microbes into routine ecological assessments. However, these target-PCR-free… read more here.
Keywords: ecological assessments; processing tools; data processing; total rna ... See more keywords
SMARTer single cell total RNA sequencing
Sign Up to like & getrecommendations! Published in 2019 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkz535
Abstract: Abstract Single cell RNA sequencing methods have been increasingly used to understand cellular heterogeneity. Nevertheless, most of these methods suffer from one or more limitations, such as focusing only on polyadenylated RNA, sequencing of only… read more here.
Keywords: single cell; rna; rna sequencing; total rna ... See more keywords
Preparation of Total RNA from Fission Yeast.
Sign Up to like & getrecommendations! Published in 2017 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot091629
Abstract: Treatment with hot phenol breaks open fission yeast cells and begins to strip away bound proteins from RNA. Deproteinization is completed by multiple extractions with chloroform/isoamyl alcohol and separation of the aqueous and organic phases… read more here.
Keywords: rna fission; fission; total rna; fission yeast ... See more keywords
Purification of Total RNA from Mammalian Cells and Tissues.
Sign Up to like & getrecommendations! Published in 2020 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot101659
Abstract: This protocol describes the use of a monophasic lysis reagent to isolate total RNA from mammalian cells (grown as either a monolayer or in suspension) or tissues. An alternative protocol for the isolation of RNA… read more here.
Keywords: purification total; rna; cells tissues; total rna ... See more keywords
Total RNA Isolation from Drosophila melanogaster.
Sign Up to like & getrecommendations! Published in 2020 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot101675
Abstract: Of the methods available for extracting total RNA from Drosophila, monophasic lysis reagents (such as TRIzol) yield consistent and reliable results and are considerably cheaper than other kit-based techniques for RNA isolation. In this protocol,… read more here.
Keywords: drosophila; rna isolation; isolation drosophila; drosophila melanogaster ... See more keywords