Development of duplex-crossed allele-specific PCR targeting of TPMT*3B and *3C using crossed allele-specific blockers to eliminate non-specific amplification.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2019.03.019
Abstract: Prospective testing for variants in the thiopurine S-methyltransferase (TPMT) is considered a key process in the development of thiopurine therapy. This testing is done to avoid toxicity and side effects in the management of diverse… read more here.
Keywords: non specific; specific amplification; crossed allele; pcr ... See more keywords
Determination of thiopurine S‐methyltransferase activity by hydrophilic interaction liquid chromatography hyphenated with mass spectrometry
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Pharmaceutical and Biomedical Analysis"
DOI: 10.1016/j.jpba.2017.05.016
Abstract: HighlightsNew HILIC‐HPLC‐MS method was developed for determination of TPMT activity.HILIC‐HPLC‐MS combination was approved by excellent performance parameters.HILIC‐HPLC‐MS was compared to traditional RP‐HPLC‐UV as well as advanced RP‐HPLC–MS.Conventional approaches were surpassed in terms of selectivity, LOD,… read more here.
Keywords: hplc; determination; tpmt; liquid chromatography ... See more keywords
Distribution of Genetic Polymorphisms of Genes Implicated in Thiopurine Drugs Metabolism
Sign Up to like & getrecommendations! Published in 2018 at "Therapeutic Drug Monitoring"
DOI: 10.1097/ftd.0000000000000548
Abstract: Abstract: Thiopurine-S-methyltransferase (TPMT) and inosine triphosphate pyrophosphatase (ITPA) are crucial enzymes involved in the metabolism of thiopurine drugs. Significant interethnic variation in the expression of TPMT and ITPA is caused by single nucleotide polymorphisms of… read more here.
Keywords: itpa; polymorphisms genes; thiopurine; tpmt ... See more keywords
Homozygote CRIM1 variant is associated with thiopurine-induced neutropenia in leukemic patients with both wildtype NUDT15 and TPMT
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Translational Medicine"
DOI: 10.1186/s12967-020-02416-7
Abstract: NUDT15 and TPMT variants are strong genetic determinants of thiopurine-induced hematological toxicity that results in therapeutic failure in pediatric acute lymphoblastic leukemia (ALL). However, many patients with both wild-type (WT) NUDT15 and TPMT still suffer… read more here.
Keywords: variant; thiopurine induced; nudt15 tpmt; tpmt ... See more keywords
Evaluation of prescriber responses to pharmacogenomics clinical decision support for thiopurine S‐methyltransferase testing
Sign Up to like & getrecommendations! Published in 2018 at "American Journal of Health-System Pharmacy"
DOI: 10.2146/ajhp170280
Abstract: Purpose Results of a study of prescribers’ responses to a pharmacogenomics‐based clinical decision support (CDS) alert designed to prompt thiopurine S‐methyltransferase (TPMT) status testing are reported. Methods A single‐center, retrospective, chart review–based study was conducted… read more here.
Keywords: responses pharmacogenomics; prescriber; decision support; thiopurine ... See more keywords
Comparison of Direct Sequencing, Real-Time PCR-High Resolution Melt (PCR-HRM) and PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) Analysis for Genotyping of Common Thiopurine Intolerant Variant Alleles NUDT15 c.415C>T and TPMT c.719A>G (TPMT*3C)
Sign Up to like & getrecommendations! Published in 2017 at "Diagnostics"
DOI: 10.3390/diagnostics7020027
Abstract: Thiopurine intolerance and treatment-related toxicity, such as fatal myelosuppression, is related to non-function genetic variants encoding thiopurine S-methyltransferase (TPMT) and Nudix hydrolase 15 (NUDT15). Genetic testing of the common variants NUDT15:NM_018283.2:c.415C>T (Arg139Cys, dbSNP rs116855232 T… read more here.
Keywords: pcr hrm; real time; time pcr; hrm pcr ... See more keywords
Thiopurine S-Methyltransferase Polymorphisms Predict Hepatotoxicity in Azathioprine-Treated Patients with Autoimmune Diseases
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Personalized Medicine"
DOI: 10.3390/jpm12091399
Abstract: Thiopurine methyltransferase (TPMT) is the rate-limiting enzyme in Azathioprine (AZA) metabolization. Although studies have discussed the association between the TPMT polymorphisms and myelosuppression, the data about the relationship between TPMT genotypes and hepatoxicity in Asian… read more here.
Keywords: thiopurine methyltransferase; non; hepatotoxicity; tpmt ... See more keywords
Genetic Variants of Pre-microRNAs A-499G(rs3746444) and T-196a2C(rs11614913) with Ulcerative Colitis (UC) and Investigated with Thiopurine-S-Methyltransferase (TPMT) Activity.
Sign Up to like & getrecommendations! Published in 2017 at "Clinical laboratory"
DOI: 10.7754/clin.lab.2017.170502
Abstract: BACKGROUND Abnormal expression and different splicing of miRNAs are involved in several human inflammatory disorders. It has been suggested that gene variants of miRNAs may be associated with increased risk of ulcerative colitis (UC). We… read more here.
Keywords: 499g rs3746444; mirna 499g; tpmt; tpmt activity ... See more keywords