Unveiling Cas12j Trans‐Cleavage Activity for CRISPR Diagnostics: Application to miRNA Detection in Lung Cancer Diagnosis
Sign Up to like & getrecommendations! Published in 2024 at "Advanced Science"
DOI: 10.1002/advs.202402580
Abstract: Cas12j, a hypercompact and efficient Cas protein, has potential for use in CRISPR diagnostics, but has not yet been used because the trans‐cleavage activity of Cas12j is veiled. Here, the trans‐cleavage behavior of Cas12j1, 2,… read more here.
Keywords: detection; crispr diagnostics; trans cleavage; cleavage activity ... See more keywords
High-Fidelity CRISPR/Cas13a trans-Cleavage-Triggered Rolling Circle Amplified DNAzyme for Visual Profiling of MicroRNA.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c03708
Abstract: The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) (CRISPR/Cas) system innovates a next-generation biosensor due to its high-fidelity, programmability, and efficient signal amplification ability. Developing a CRISPR/Cas-based visual detection system could contribute… read more here.
Keywords: high fidelity; crispr cas13a; detection; trans cleavage ... See more keywords
"RESET" Effect: Random Extending Sequences Enhance the Trans-Cleavage Activity of CRISPR/Cas12a.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c01401
Abstract: The trans-cleavage activity of CRISPR/Cas12a has been widely used in biosensing applications. However, the lack of exploration on the fundamental properties of CRISPR/Cas12a not only discourages further in-depth studies of the CRISPR/Cas12a system but also… read more here.
Keywords: crispr cas12a; trans cleavage; cas12a; cleavage activity ... See more keywords
Signal Amplification by the trans-Cleavage Activity of CRISPR-Cas Systems: Kinetics and Performance
Sign Up to like & getrecommendations! Published in 2023 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.2c04555
Abstract: ACCESS Metrics & More Article Recommendations ■ CONTENTS Functional Basics of Cas12 and Cas13 208 Nuclease Activity of Cas12a 208 Nuclease Activity of Cas13a 208 Quantitative Determination of trans-Cleavage Kinetics of Cas Enzymes 209 trans-Cleavage… read more here.
Keywords: signal amplification; trans cleavage; cleavage kinetics; activity ... See more keywords
Synergistic Incorporation of Two ssDNA Activators Enhances the Trans-Cleavage of CRISPR/Cas12a.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.3c00414
Abstract: CRISPR/Cas12a has been believed to be powerful in molecular detection and diagnostics due to its amplified trans-cleavage feature. However, the activating specificity and multiple activation mechanisms of the Cas12a system are yet to be elucidated… read more here.
Keywords: incorporation two; trans cleavage; cas12a; ssdna activators ... See more keywords
Exploring the Effect of Steric Hindrance on Trans-cleavage Activity of CRISPR-cas12a for Ultrasensitive SERS Detection of P53 DNA.
Sign Up to like & getrecommendations! Published in 2024 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.4c01347
Abstract: The trans-cleavage properties of Cas12a make it important for gene editing and disease diagnosis. In this work, the effect of spatial site resistance on the trans-cleavage activity of Cas12a was studied. First, we have explored… read more here.
Keywords: trans cleavage; cleavage activity; effect; cas12a ... See more keywords
A Cascade Signal Amplification Based on Dynamic DNA Nanodevices and CRISPR/Cas12a Trans-cleavage for Highly Sensitive MicroRNA Sensing.
Sign Up to like & getrecommendations! Published in 2021 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.1c00064
Abstract: The variations of microRNA (miRNA) expression can be valuable biomarkers in disease diagnosis and prognosis. However, current miRNA detection techniques mainly rely on reverse transcription and template replication, which suffer from slowness, contamination risk, and… read more here.
Keywords: crispr cas12a; dna; trans cleavage; highly sensitive ... See more keywords
One-Pot Detection of Biomarker Apurinic/Apyrimidinic Endonuclease 1 Based on the Modified-crRNA Regulated Trans-Cleavage Activity of CRISPR/Cas12a.
Sign Up to like & getrecommendations! Published in 2025 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.5c00335
Abstract: Apurinic/apyrimidinic endonuclease 1 (APE1), a critical protein in DNA repair, plays indispensable roles in the maintenance of cellular homeostasis, thereby garnering significant attention as a biomarker and therapeutic target for various disorders. Current APE1 sensing… read more here.
Keywords: detection; trans cleavage; apyrimidinic endonuclease; apurinic apyrimidinic ... See more keywords
Next-generation CRISPR/Cas-based ultrasensitive diagnostic tools: current progress and prospects
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DOI: 10.1039/d4ra04838e
Abstract: CRISPR/Cas has been explored as a powerful molecular scissor that uses a double-strand break mediated non-homologous end joining (NHEJ) or homology-directed repair (HDR) to achieve precise gene editing. Cas effectors come in several different forms,… read more here.
Keywords: crispr; trans cleavage; crispr cas; next generation ... See more keywords
Enhancement of CRISPR/Cas12a trans-cleavage activity using hairpin DNA reporters
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac578
Abstract: Abstract The RNA programmed non-specific (trans) nuclease activity of CRISPR-Cas Type V and VI systems has opened a new era in the field of nucleic acid-based detection. Here, we report on the enhancement of trans-cleavage… read more here.
Keywords: hairpin dna; dna; trans cleavage; cleavage activity ... See more keywords