An Extended Approach for the Development of Fluorogenic trans‐Cyclooctene–Tetrazine Cycloadditions
Sign Up to like & getrecommendations! Published in 2019 at "Chembiochem"
DOI: 10.1002/cbic.201800711
Abstract: Inverse‐electron‐demand Diels–Alder (iEDDA) cycloaddition between 1,2,4,5‐tetrazines and strained dienophiles belongs among the most popular bioconjugation reactions. In addition to its fast kinetics, this cycloaddition can be tailored to produce fluorescent products from non‐fluorescent starting materials.… read more here.
Keywords: cycloaddition; extended approach; iedda cycloaddition; trans cyclooctene ... See more keywords
[11 C]Carboxylated Tetrazines for Facile Labeling of Trans-Cyclooctene-Functionalized PeptoBrushes.
Sign Up to like & getrecommendations! Published in 2021 at "Macromolecular rapid communications"
DOI: 10.1002/marc.202100655
Abstract: Functionalization of macromolecules (antibodies, polymers, nanoparticles) with click-reactive groups greatly enhances the versatility of their potential applications. Click chemistry based on tetrazine - trans-cyclooctene (TCO) ligation is especially promising and is already widely applied for… read more here.
Keywords: trans cyclooctene; tetrazines facile; facile labeling; labeling trans ... See more keywords
A Versatile Microarray Immobilization Strategy Based on a Biorthogonal Reaction Between Tetrazine and Trans-Cyclooctene.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6584-7_6
Abstract: Given its increasing importance in transforming biomedical research in recent years, microarray technology has become highly popular as a powerful screening platform in detecting biomolecule interactions, discovering new inhibitors, and identifying biomarkers as well as… read more here.
Keywords: microarray; immobilization; microarray immobilization; immobilization strategy ... See more keywords
trans-Cyclooctene- and Bicyclononyne-Linked Nucleotides for Click Modification of DNA with Fluorogenic Tetrazines and Live Cell Metabolic Labeling and Imaging
Sign Up to like & getrecommendations! Published in 2023 at "Bioconjugate Chemistry"
DOI: 10.1021/acs.bioconjchem.3c00064
Abstract: A series of 2′-deoxyribonucleoside triphosphates (dNTPs) bearing 2- or 4-linked trans-cyclooctene (TCO) or bicyclononyne (BCN) tethered through a shorter propargylcarbamate or longer triethyleneglycol-based spacer were designed and synthesized. They were found to be good substrates… read more here.
Keywords: trans cyclooctene; dna; bicyclononyne; metabolic labeling ... See more keywords
Easy Access to Bioorthogonal Click-to-Release Reagent Bishydroxy-trans-cyclooctene (C2TCO) and Harnessing of Its Rapid Labeling and Dissecting Feature in Multicycle Imaging.
Sign Up to like & getrecommendations! Published in 2025 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.4c00495
Abstract: Bifunctional trans-cyclooctene (bTCO) with a carbamate or carbonate at the allylic position and tetrazine provide a promising bioorthogonal click chemistry pair for the click-to-release approach, successfully employed in various biotechnological applications. Herein, we demonstrate a… read more here.
Keywords: chemistry; bioorthogonal click; click; click release ... See more keywords
Trans-Cyclooctene Isomerization Catalyzed by Thiamine Degradation Products in Cell Culture Media
Sign Up to like & getrecommendations! Published in 2025 at "ACS Omega"
DOI: 10.1021/acsomega.5c01780
Abstract: PET imaging of intrathecally dosed ASO neurology drugs is challenging due to the long time needed to achieve steady-state brain distribution, making the use of a simple 18F tag impossible with its short radioactive half-life.… read more here.
Keywords: thiamine degradation; cell culture; culture media; culture ... See more keywords
Computation-Guided Discovery of Diazole Monosubstituted Tetrazines as Optimal Bioorthogonal Tools.
Sign Up to like & getrecommendations! Published in 2024 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.4c07958
Abstract: Monosubstituted tetrazines are important bioorthogonal reactive tools due to their rapid ligation with trans-cyclooctene. However, their application is limited by the reactivity-stability paradox in biological environments. In this study, we demonstrated that steric effects are… read more here.
Keywords: computation guided; guided discovery; trans cyclooctene; monosubstituted tetrazines ... See more keywords