An Extended Approach for the Development of Fluorogenic trans‐Cyclooctene–Tetrazine Cycloadditions
Sign Up to like & getrecommendations! Published in 2019 at "Chembiochem"
DOI: 10.1002/cbic.201800711
Abstract: Inverse‐electron‐demand Diels–Alder (iEDDA) cycloaddition between 1,2,4,5‐tetrazines and strained dienophiles belongs among the most popular bioconjugation reactions. In addition to its fast kinetics, this cycloaddition can be tailored to produce fluorescent products from non‐fluorescent starting materials.… read more here.
Keywords: cycloaddition; extended approach; iedda cycloaddition; trans cyclooctene ... See more keywords
[11 C]Carboxylated Tetrazines for Facile Labeling of Trans-Cyclooctene-Functionalized PeptoBrushes.
Sign Up to like & getrecommendations! Published in 2021 at "Macromolecular rapid communications"
DOI: 10.1002/marc.202100655
Abstract: Functionalization of macromolecules (antibodies, polymers, nanoparticles) with click-reactive groups greatly enhances the versatility of their potential applications. Click chemistry based on tetrazine - trans-cyclooctene (TCO) ligation is especially promising and is already widely applied for… read more here.
Keywords: trans cyclooctene; tetrazines facile; facile labeling; labeling trans ... See more keywords
A Versatile Microarray Immobilization Strategy Based on a Biorthogonal Reaction Between Tetrazine and Trans-Cyclooctene.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6584-7_6
Abstract: Given its increasing importance in transforming biomedical research in recent years, microarray technology has become highly popular as a powerful screening platform in detecting biomolecule interactions, discovering new inhibitors, and identifying biomarkers as well as… read more here.
Keywords: microarray; immobilization; microarray immobilization; immobilization strategy ... See more keywords
trans-Cyclooctene- and Bicyclononyne-Linked Nucleotides for Click Modification of DNA with Fluorogenic Tetrazines and Live Cell Metabolic Labeling and Imaging
Sign Up to like & getrecommendations! Published in 2023 at "Bioconjugate Chemistry"
DOI: 10.1021/acs.bioconjchem.3c00064
Abstract: A series of 2′-deoxyribonucleoside triphosphates (dNTPs) bearing 2- or 4-linked trans-cyclooctene (TCO) or bicyclononyne (BCN) tethered through a shorter propargylcarbamate or longer triethyleneglycol-based spacer were designed and synthesized. They were found to be good substrates… read more here.
Keywords: trans cyclooctene; dna; bicyclononyne; metabolic labeling ... See more keywords