Heterodimerization of Group I Ribozymes Enabling Exon Recombination through Pairs of Cooperative trans‐Splicing Reactions
Sign Up to like & getrecommendations! Published in 2017 at "ChemBioChem"
DOI: 10.1002/cbic.201700053
Abstract: Group I (GI) self‐splicing ribozymes are attractive tools for biotechnology and synthetic biology. Several trans‐splicing and related reactions based on GI ribozymes have been developed for the purpose of recombining their target mRNA sequences. By combining… read more here.
Keywords: cooperative trans; group; rational modular; splicing reactions ... See more keywords
Therapeutic applications of group I intron‐based trans‐splicing ribozymes
Sign Up to like & getrecommendations! Published in 2018 at "Wiley Interdisciplinary Reviews: RNA"
DOI: 10.1002/wrna.1466
Abstract: Since the breakthrough discovery of catalytic RNAs (ribozymes) in the early 1980s, valuable ribozyme‐based gene therapies have been developed for incurable diseases ranging from genetic disorders to viral infections and cancers. Ribozymes can be engineered… read more here.
Keywords: splicing ribozymes; group intron; gene; trans splicing ... See more keywords
LOV2-Controlled Photoactivation of Protein Trans-Splicing.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6451-2_15
Abstract: Protein trans-splicing is a posttranslational modification that joins two protein fragments together via a peptide a bond in a process that does not require exogenous cofactors. Towards achieving cellular control, synthetically engineered systems have used… read more here.
Keywords: protein; controlled photoactivation; lov2 controlled; protein trans ... See more keywords
Promiscuous Trans-splicing Activities Revealed by Next Generation Sequencing-based Analysis of 298 Split Inteins
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology and Bioprocess Engineering"
DOI: 10.1007/s12257-019-0394-x
Abstract: Protein trans-splicing is a naturally occurring process in which two protein fragments are ligated by a reaction between two intein domains, called split inteins. Despite their usefulness in research, the reactivity and structure of only… read more here.
Keywords: split inteins; split; splicing activities; next generation ... See more keywords
A high-throughput screen for the identification of compounds that inhibit nematode gene expression by targeting spliced leader trans-splicing
Sign Up to like & getrecommendations! Published in 2019 at "International Journal for Parasitology: Drugs and Drug Resistance"
DOI: 10.1016/j.ijpddr.2019.04.001
Abstract: Infections with parasitic nematodes are among the most significant of the neglected tropical diseases affecting about a billion people living mainly in tropical regions with low economic activity. The most effective current strategy to control… read more here.
Keywords: leader trans; spliced leader; anthelmintic drugs; trans splicing ... See more keywords
Efficient generation of bispecific IgG antibodies by split intein mediated protein trans-splicing system
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-08641-3
Abstract: Many methods have been developed to produce bispecific antibodies (BsAbs) for industrial application. However, huge challenges still remain in synthesizing whole length BsAbs, including their assembly, stability, immunogenicity, and pharmacodynamics. Here we present for first… read more here.
Keywords: bispecific igg; split intein; protein trans; igg antibodies ... See more keywords
slORFfinder: a tool to detect open reading frames resulting from trans-splicing of spliced leader sequences
Sign Up to like & getrecommendations! Published in 2023 at "Briefings in Bioinformatics"
DOI: 10.1093/bib/bbac610
Abstract: Abstract Trans-splicing of a spliced leader (SL) to the 5′ ends of mRNAs is used to produce mature mRNAs in several phyla of great importance to human health and the marine ecosystem. One of the… read more here.
Keywords: reading frames; tool; trans splicing; spliced leader ... See more keywords
SL-quant: a fast and flexible pipeline to quantify spliced leader trans-splicing events from RNA-seq data
Sign Up to like & getrecommendations! Published in 2018 at "GigaScience"
DOI: 10.1093/gigascience/giy084
Abstract: Abstract Background The spliceosomal transfer of a short spliced leader (SL) RNA to an independent pre-mRNA molecule is called SL trans-splicing and is widespread in the nematode Caenorhabditis elegans. While RNA-sequencing (RNA-seq) data contain information… read more here.
Keywords: quant fast; spliced leader; seq data; rna seq ... See more keywords
An in vivo genetic screen for genes involved in spliced leader trans-splicing indicates a crucial role for continuous de novo spliced leader RNP assembly
Sign Up to like & getrecommendations! Published in 2017 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkx500
Abstract: Abstract Spliced leader (SL) trans-splicing is a critical element of gene expression in a number of eukaryotic groups. This process is arguably best understood in nematodes, where biochemical and molecular studies in Caenorhabditis elegans and… read more here.
Keywords: leader trans; spliced leader; vivo genetic; role ... See more keywords
Trans-splicing enhances translational efficiency in C. elegans.
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DOI: 10.1101/gr.202150.115
Abstract: Translational efficiency is subject to extensive regulation. However, the factors influencing such regulation are poorly understood. In Caenorhabditis elegans, 62% of genes are trans-spliced to a specific spliced leader (SL1), which replaces part of the… read more here.
Keywords: enhances translational; trans spliced; translational efficiency; sl1 trans ... See more keywords
Hypothesis: Trans‐splicing Generates Evolutionary Novelty in the Photosynthetic Amoeba Paulinella
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Phycology"
DOI: 10.1111/jpy.13247
Abstract: Plastid primary endosymbiosis has occurred twice, once in the Archaeplastida ancestor and once in the Paulinella (Rhizaria) lineage. Both events precipitated massive evolutionary changes, including the recruitment and activation of genes that are horizontally acquired… read more here.
Keywords: hypothesis trans; generates evolutionary; trans splicing; paulinella ... See more keywords