Directed Evolution of an (R)‐Selective Transaminase Toward Higher Efficiency of Sitagliptin Analog Biosynthesis
Sign Up to like & getrecommendations! Published in 2025 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28988
Abstract: Transaminase (TA)‐catalyzed asymmetric amination is considered as a green chemistry approach to synthesize pharmaceutical analogs, but their ability to accept substrate for catalyzing sterically hindered ketones remains a challenge. Sitagliptin is an antihyperglycemic drug to… read more here.
Keywords: transaminase; sitagliptin; sitagliptin analog; directed evolution ... See more keywords
Raman spectroscopy and one‐dimensional convolutional neural network modeling as a real‐time monitoring tool for in vitro transaminase‐catalyzed synthesis of a pharmaceutically relevant amine precursor
Sign Up to like & getrecommendations! Published in 2024 at "Biotechnology Progress"
DOI: 10.1002/btpr.3444
Abstract: Raman spectroscopy has been used to measure the concentration of a pharmaceutically relevant model amine intermediate for positive allosteric modulators of nicotinic acetylcholine receptor in a ω‐transaminase‐catalyzed conversion. A model based on a one‐dimensional convolutional… read more here.
Keywords: amine; spectroscopy; transaminase; raman spectroscopy ... See more keywords
Modulation of Transaminase Activity by Encapsulation in Temperature‐Sensitive Poly(N‐acryloyl glycinamide) Hydrogels
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DOI: 10.1002/cbic.202100427
Abstract: Smart hydrogels hold much potential for biocatalysis, not only for the immobilization of enzymes, but also for the control of enzyme activity. We investigated upper critical solution temperature‐type poly N‐acryloyl glycinamide (pNAGA) hydrogels as a… read more here.
Keywords: transaminase; acryloyl glycinamide; temperature; activity ... See more keywords
Recombinant l‐Amino Acid Oxidase with Broad Substrate Spectrum for Co‐substrate Recycling in (S)‐Selective Transaminase‐Catalyzed Kinetic Resolutions
Sign Up to like & getrecommendations! Published in 2022 at "Chembiochem"
DOI: 10.1002/cbic.202200329
Abstract: Chiral and enantiopure amines can be produced by enantioselective transaminases via kinetic resolution of amine racemates. This transamination reaction requires stoichiometric amounts of co‐substrate. A dual‐enzyme recycling system overcomes this limitation: l‐amino acid oxidases (LAAO)… read more here.
Keywords: substrate spectrum; amino acid; transaminase; substrate ... See more keywords
Improving Catalytic Activity and Reversing Enantio‐Specificity of ω‐Transaminase by Semi‐Rational Engineering en Route to Chiral Bulky β‐Amino Esters
Sign Up to like & getrecommendations! Published in 2021 at "ChemCatChem"
DOI: 10.1002/cctc.202100503
Abstract: The application of wild‐type ω‐transaminase was limited by steric hindrance towards bulky substrates, therefore improvement of the catalytic efficiency and stereoselectivity toward substrates with two bulky substituent adjacent to the carbonyl is of general interest.… read more here.
Keywords: bulky; keto esters; reversing enantio; transaminase ... See more keywords
Continuous Preparative Application of Amine Transaminase‐Catalyzed Reactions with Integrated Crystallization
Sign Up to like & getrecommendations! Published in 2024 at "European Journal of Organic Chemistry"
DOI: 10.1002/ejoc.202400068
Abstract: Transaminases are valuable catalysts in the synthesis of chiral amines. However, secondary techniques are typically required to shift the reaction equilibrium to the product side. This study focuses on the in situ‐product crystallization, specifically at… read more here.
Keywords: transaminase; crystallization; product; preparative application ... See more keywords
The De Ritis (aspartate transaminase/alanine transaminase) ratio as a predictor of oncological outcomes in patients after surgery for upper urinary tract urothelial carcinoma
Sign Up to like & getrecommendations! Published in 2017 at "International Urology and Nephrology"
DOI: 10.1007/s11255-017-1613-z
Abstract: PurposeRecently, several studies have shown that the De Ritis ratio (aspartate transaminase/alanine transaminase) can be a useful prognostic biomarker for certain types of malignant tumors. However, the prognostic value of the De Ritis ratio in… read more here.
Keywords: ritis ratio; tract urothelial; ratio; transaminase ... See more keywords
The significance of De Ritis (aspartate transaminase/alanine transaminase) ratio in predicting pathological outcomes and prognosis in localized prostate cancer patients
Sign Up to like & getrecommendations! Published in 2017 at "International Urology and Nephrology"
DOI: 10.1007/s11255-017-1618-7
Abstract: PurposeTo illustrate whether De Ritis (aspartate transaminase-AST/alanine transaminase-ALT) ratio is useful in risk stratification of localized prostate cancer and propose an easy predictive model for biochemical recurrence-free survival (BCRFS).MethodsIn total, 438 patients who underwent radical… read more here.
Keywords: localized prostate; prostate cancer; ratio; transaminase ... See more keywords
Enhanced conversion of biomass to furfurylamine with high productivity by tandem catalysis with sulfonated perlite and ω-transaminase whole-cell biocatalyst.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of biotechnology"
DOI: 10.1016/j.jbiotec.2021.05.003
Abstract: Production of bio-based chemicals from renewable bioresource is a key driver for moving towards sustainable industry. Furfurylamine is known as an important furfural-upgrading product in organic synthesis, as well as monolithic synthetic pharmaceuticals, fibers, additives… read more here.
Keywords: biomass; furfurylamine high; biocatalyst; furfurylamine ... See more keywords
The effect of supplementation with Scutellaria baicalensis on hepatic function.
Sign Up to like & getrecommendations! Published in 2019 at "Medical hypotheses"
DOI: 10.1016/j.mehy.2019.109402
Abstract: The dried root of the angiosperm Scutellaria baicalensis, also known as Chinese skullcap or Baikal skullcap, is widely used in traditional Chinese medicine, Korean traditional medicine and as a nutritional supplement; several studies have indicated… read more here.
Keywords: medicine; supplementation; scutellaria baicalensis; supplementation scutellaria ... See more keywords
Transaminase encoded by NCgl2515 gene of Corynebacterium glutamicum ATCC13032 is involved in γ-aminobutyric acid decomposition
Sign Up to like & getrecommendations! Published in 2017 at "Process Biochemistry"
DOI: 10.1016/j.procbio.2017.01.016
Abstract: Abstract Corynebacterium glutamicum that expresses an exogenous l -glutamate decarboxylase (GAD) gene can synthesize γ-aminobutyric acid (GABA). GABA is decomposed to succinic semialdehyde (SSA) by GABA transaminase (GABA-T) and to succinate thereafter by SSA dehydrogenase… read more here.
Keywords: gabt; glutamicum; gaba; transaminase ... See more keywords