Purification of Zygotically Transcribed RNA through Metabolic Labeling of Early Zebrafish Embryos.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6988-3_8
Abstract: Early embryonic development in all known metazoans is characterized by a transcriptionally silent phase, during which development is under control of maternally loaded protein and RNA. The zygotic genome becomes transcriptionally active after a series… read more here.
Keywords: transcribed rna; embryos; zebrafish embryos; zygotically transcribed ... See more keywords
Capturing the interactome of newly transcribed RNA
Sign Up to like & getrecommendations! Published in 2018 at "Nature Methods"
DOI: 10.1038/nmeth.4595
Abstract: We combine the labeling of newly transcribed RNAs with 5-ethynyluridine with the characterization of bound proteins. This approach, named capture of the newly transcribed RNA interactome using click chemistry (RICK), systematically captures proteins bound to… read more here.
Keywords: transcribed rna; newly transcribed; interactome newly; chemistry ... See more keywords
Translation of in vitro-transcribed RNA therapeutics
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2023.1128067
Abstract: In vitro transcribed, modified messenger RNAs (IVTmRNAs) have been used to vaccinate billions of individuals against the SARS-CoV-2 virus, and are currently being developed for many additional therapeutic applications. IVTmRNAs must be translated into proteins… read more here.
Keywords: vitro; vitro transcribed; rna therapeutics; translation vitro ... See more keywords
Nucleotide Context Can Modulate Promoter Strength in Genes Transcribed by RNA Polymerase III
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DOI: 10.3390/genes14040802
Abstract: The small nuclear RNAs 4.5SH and 4.5SI were characterized only in mouse-like rodents; their genes originate from 7SL RNA and tRNA, respectively. Similar to many genes transcribed by RNA polymerase III (pol III), the genes… read more here.
Keywords: promoter; strength; iii; genes transcribed ... See more keywords